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- W2336702704 abstract "OBJECTIVE The methylation of RAS associated domain family 1A gene(RASSF1A)and RASSF2 A were found in incidence and progress of many kinds of tumor,and RASSF1 A was considered as biological indicator of ovarian cancer.The objective of this study is to evaluate the methylation status of RASSF2 Ain epithelial ovarian cancer(EOC)tissues,and its correlation with expression level of RASSF2 Fand clinicopathologic features.METHODS The sample sources were from Liaocheng People’s Hospital from October 1 2013 to December 31 2014.Methylation-specific PCR was used to detect the methylation status of RASSF2 Agene in tissues from 50 EOC patients,27 patients with borderline epithelial ovarian neoplasms and 20 patients with benign epithelial ovarian tumors.The gene expression of mRNA was examined by RT-PCR.The EOC cell lines,SKOV3 and 3AO were treated with 5-aza-2′-deoxycytidine(5-aza-dC).RESULTS The frequency of aberrant methylation of RASSF2 Awas 46.00%(23/50)in EOC tissues and 22.22%(6/27)in borderline epithelial ovarian neoplasms samples,whereas such hypermethylation was not detected in benign epithelial ovarian tumors(χ2=15.474,P<0.001).In EOC,there were no significant correlations between the promoter hypermethylated status of the RASSF2 Aand clinicopathologic characteristics,such as age,FIGO stage,type of tumor,histopathologic grade and lymph node metastasis.The rates of RASSF2 A mRNA expression were 95.00%(19/20),59.26%(16/27),34.00%(17/50)in above ovarian tissues,repectively(χ2=21.855,P<0.001).The expression of RASSF2 A mRNA was significantly down-regulated or lost in the methylated group compared to the unmethylated group.RASSF2 A promoter methylation was negatively correlated with its mRNA expression.After treatment with 5-aza-dC,promoter methylation status of ovarian cancer cell lines(Skov3and 3AO)was reversed,RASSF2 A mRNA expression was significantly restored in the cell lines.CONCLUSION Epigenetic inactivation of RASSF2 Athrough aberrant promoter methylation may play an important role in the oncogenesis and progression of EOC." @default.
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- W2336702704 date "2015-01-01" @default.
- W2336702704 modified "2023-09-22" @default.
- W2336702704 title "Methylation and expression of RASSF2A in epithelial ovarian cancer tissues" @default.
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