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- W2336952020 abstract "Objective To investigate the effect of meglumine cyclic adenylate(MCA) on the expression of cAMP response element binding protein(CREB) in myocardial tissue of piglets with hypoxic-ischemic encephalopathy(HIE).Methods 22 term piglets at 3 to 7 days after birth were subjected and divided into sham operation group(4),HIE(9,block bilateral common carotid artery temporarily and give 6%oxygen mechanically) group and MCA(9,injection of MCA in vein before ischemia)group.The content of cardial troponin I in myocardium was measured by chemiluminescence,myocardial cell structure changes was observed by electron microscope,the expression of CREB protein and mRNA was detected by Western blot and Real-time PCR method respectively.Results The content of cardial troponin I in myocardium was significantly higher in HIE group than in sham group or MCA group 10 hours after hypoxia(P <0.01).The cellular structure of myocardium was intact in sham group,but injured severely in HIE group,milder in MCA group.Compared with,The expression levels of CREB protein and mRNA in myocardial tissue were decreased in HIE group than in sham group,but increased significantly in MCA group than in HIE group(P<0.01).Conclusion MCA could up-regulate the expression of CREB in myocardial tissue of neonatal piglets with hypoxic-ischemic encephalopathy." @default.
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- W2336952020 date "2016-01-01" @default.
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- W2336952020 title "The effect of meglumine cyclic adenylate on the expression of CREB in myocardial tissue of piglets with hypoxic-ischemic encephalopathy" @default.
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