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- W2337128228 abstract "The plant germplasm collections represent an important way for ex situ conservation of plant species on short, medium or long-term period of time. In order to establish a reproducible protocol for the introduction to in vitro gene bank collection based on tissue cultures of two rare taxa -Dianthus spiculifolius and D. glacialis ssp. gelidus, morphological, developmental and biochemical changes (antioxidant enzymes and protein spectra) after the prolonged culture in the presence of three levels of mannitol (0.16 M, 0.32 M and 0.49 M) as growth retardant were evaluated. Our results showed the positive effect of the mannitol for in vitro conservation in restricted growth conditions, its presence being compatible with the plant survival and the regeneration capacity. An important aspect was the induction and development of somatic embryos in the presence of mannitol as osmotic active factor. The extended culture in the minimal growth conditions, determined the increase of antioxidant enzyme activity and some small variations in enzyme spectra as peroxidase, superoxid dismutase, catalase and protein patterns. The alterations in the proteins and enzymes pattern comparing to the control can be associated to the prolonged culture, to the desiccation stress, to the adaptation mechanisms as response to stress and developmental processes." @default.
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- W2337128228 date "2010-01-01" @default.
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- W2337128228 title "The establishment of an in vitro gene bank in Dianthus spiculifolius Schur. and D. glacialis ssp. gelidus (Schott Nym. et Kotschy) Tutin: II. Mediumterm cultures characterization in minimal growth conditions." @default.
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