Matches in SemOpenAlex for { <https://semopenalex.org/work/W2337172895> ?p ?o ?g. }
- W2337172895 abstract "The necessary multi-step process to prepare human semen for assisted reproduction (AR) such as in vitro fertilization (IVF) is known to induce oxidative stress in human spermatozoa and subsequent damage to the integrity of their cell components and, thus, to their function. Literature reports have viewed this as a potential link with the high frequency of birth defects among the IVF babies. In this work, we studied the effect of incubation and centrifugation on human sperm quality using established AR protocols. We found that in vitro incubation and centrifugation (20 min at 220g) of sperm generally degrade sperm quality in terms of motility and DNA oxidation. The total antioxidant capacity of the semen significantly decreased upon raising the centrifugation force from 220g to 400g. We then studied the efficacy of supplements such as L-carnitine (LC) on remediating the oxidative stress. We found that supplementation with LC counteracts the overall damage of quality brought by the multi-step method of sperm processing. We determined the LC concentration range (0.1-1.0 mg/mL) with optimum oxidative stress remediation and which enhances qualities such as motility. Among the various oxidative stress species, we particularly focused on nitric oxide as a species with known dual functional and cytotoxic properties. We have demonstrated a method to monitor its dynamic concentrations in spermatozoa. The method enabled us to establish a link between motility and nitric oxide levels in spermatozoa. The method also allowed us to" @default.
- W2337172895 created "2016-06-24" @default.
- W2337172895 creator A5020454372 @default.
- W2337172895 creator A5021697745 @default.
- W2337172895 creator A5064314987 @default.
- W2337172895 date "2011-01-01" @default.
- W2337172895 modified "2023-09-27" @default.
- W2337172895 title "Semen Quality and Chemical Oxidative Stress; Quantification and Remediation" @default.
- W2337172895 cites W1505324034 @default.
- W2337172895 cites W1505704931 @default.
- W2337172895 cites W1507501714 @default.
- W2337172895 cites W1532056548 @default.
- W2337172895 cites W1552348327 @default.
- W2337172895 cites W1630502818 @default.
- W2337172895 cites W1764308599 @default.
- W2337172895 cites W1892561240 @default.
- W2337172895 cites W1897849704 @default.
- W2337172895 cites W1905493157 @default.
- W2337172895 cites W1964289450 @default.
- W2337172895 cites W1968632157 @default.
- W2337172895 cites W1969941249 @default.
- W2337172895 cites W1969942783 @default.
- W2337172895 cites W1970038359 @default.
- W2337172895 cites W1972211689 @default.
- W2337172895 cites W1972254081 @default.
- W2337172895 cites W1973032240 @default.
- W2337172895 cites W1977192527 @default.
- W2337172895 cites W1978423213 @default.
- W2337172895 cites W1979272210 @default.
- W2337172895 cites W1979646361 @default.
- W2337172895 cites W198085569 @default.
- W2337172895 cites W1982060683 @default.
- W2337172895 cites W1982422614 @default.
- W2337172895 cites W1982707744 @default.
- W2337172895 cites W1990634441 @default.
- W2337172895 cites W1992544322 @default.
- W2337172895 cites W1995192577 @default.
- W2337172895 cites W1999649376 @default.
- W2337172895 cites W2000656455 @default.
- W2337172895 cites W2005916689 @default.
- W2337172895 cites W2007400083 @default.
- W2337172895 cites W2010055809 @default.
- W2337172895 cites W2015203656 @default.
- W2337172895 cites W2016704244 @default.
- W2337172895 cites W2017560333 @default.
- W2337172895 cites W2018076136 @default.
- W2337172895 cites W2018118901 @default.
- W2337172895 cites W2019814232 @default.
- W2337172895 cites W2021336175 @default.
- W2337172895 cites W2032402373 @default.
- W2337172895 cites W2034445899 @default.
- W2337172895 cites W2035246932 @default.
- W2337172895 cites W2035601862 @default.
- W2337172895 cites W2036103241 @default.
- W2337172895 cites W2039073698 @default.
- W2337172895 cites W2040260661 @default.
- W2337172895 cites W2041188865 @default.
- W2337172895 cites W2042462933 @default.
- W2337172895 cites W2044095439 @default.
- W2337172895 cites W2045918988 @default.
- W2337172895 cites W2046176885 @default.
- W2337172895 cites W2046735516 @default.
- W2337172895 cites W2052036604 @default.
- W2337172895 cites W2056147576 @default.
- W2337172895 cites W2056725674 @default.
- W2337172895 cites W2057841877 @default.
- W2337172895 cites W2057914771 @default.
- W2337172895 cites W2068301733 @default.
- W2337172895 cites W2069297991 @default.
- W2337172895 cites W2075511108 @default.
- W2337172895 cites W2076839694 @default.
- W2337172895 cites W2077941478 @default.
- W2337172895 cites W2078600646 @default.
- W2337172895 cites W2080054498 @default.
- W2337172895 cites W2082521446 @default.
- W2337172895 cites W2083332812 @default.
- W2337172895 cites W2090307308 @default.
- W2337172895 cites W2093173068 @default.
- W2337172895 cites W2095798342 @default.
- W2337172895 cites W2096634997 @default.
- W2337172895 cites W2098520172 @default.
- W2337172895 cites W2099380916 @default.
- W2337172895 cites W2100345012 @default.
- W2337172895 cites W2102848870 @default.
- W2337172895 cites W2103466421 @default.
- W2337172895 cites W2104272429 @default.
- W2337172895 cites W2109469491 @default.
- W2337172895 cites W2109709649 @default.
- W2337172895 cites W2110080661 @default.
- W2337172895 cites W2110260707 @default.
- W2337172895 cites W2115153703 @default.
- W2337172895 cites W2115536254 @default.
- W2337172895 cites W2116639989 @default.
- W2337172895 cites W2119005367 @default.
- W2337172895 cites W2120253420 @default.
- W2337172895 cites W2120807382 @default.
- W2337172895 cites W2122386753 @default.
- W2337172895 cites W2122518347 @default.
- W2337172895 cites W2125398722 @default.
- W2337172895 cites W2127997721 @default.