Matches in SemOpenAlex for { <https://semopenalex.org/work/W2337194523> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2337194523 abstract "The role of small and intermediate conductance calcium-activated potassium channels (SK and IK channels) in dorsal root ganglion (DRG) neurones was examined. Sixteen antibodies raised against human or rat SK/IK channel peptide epitopes were tested for their ability to stain cells expressing channel protein. Of sixteen antibodies, 12 (6 to SKI, 1 to SK2, 2 to SK3 and 3 to IK) were deemed suitable for immunohistochemistry in human or rat tissue. Real-time quantitative PCR (qPCR) of rat DRG cDNA was performed to examine SK/IK expression levels. DRG neurones produce mRNA for all SK/IK channels and these mRNA levels were found to increase during development. Antibody staining experiments using DRG neurones cultured from different aged animals produced a positive stain with the anti-SK3 antibody only. The number of cells that stained positively and the intensity of staining for SK3 increased with age. To investigate possible functional roles for SK/IK channels sensory neurones, action potential afterhyperpolarisations (AHPs) were recorded from cultured DRG and nodose cells. The majority of these AHPs proved to be insensitive to the SK channel blocker UCL 1848. Attempts to block medium duration AHPs in DRG cells using IK and calcium channel blockers, also failed in most cases, suggesting that some other potassium conductances) are responsible. The possibility that SK3 is functional at the terminals of primary afferents was examined next. Spinal cord slices stained with SK/EK channel antibodies revealed positive SK3 staining in the outer laminae of the dorsal horn, where small and large diameter DRG fibres are expected to terminate. In vivo experiments (done by Dr Rie Suzuki, Department of Pharmacology, UCL) using UCL 1848 and l-ethyl-2- benzimidazolinone (1-EBIO an SK channel opener) showed that SK channels are likely to be active at these terminals where they have a functional role in mediating innocuous mechanical and nociceptive responses." @default.
- W2337194523 created "2016-06-24" @default.
- W2337194523 creator A5070324108 @default.
- W2337194523 date "2005-01-01" @default.
- W2337194523 modified "2023-09-26" @default.
- W2337194523 title "A study of small and intermediate conductance calcium-activated potassium channels in sensory neurones." @default.
- W2337194523 hasPublicationYear "2005" @default.
- W2337194523 type Work @default.
- W2337194523 sameAs 2337194523 @default.
- W2337194523 citedByCount "0" @default.
- W2337194523 crossrefType "dissertation" @default.
- W2337194523 hasAuthorship W2337194523A5070324108 @default.
- W2337194523 hasConcept C126322002 @default.
- W2337194523 hasConcept C134018914 @default.
- W2337194523 hasConcept C142724271 @default.
- W2337194523 hasConcept C149614440 @default.
- W2337194523 hasConcept C149786707 @default.
- W2337194523 hasConcept C153911025 @default.
- W2337194523 hasConcept C159654299 @default.
- W2337194523 hasConcept C169760540 @default.
- W2337194523 hasConcept C170493617 @default.
- W2337194523 hasConcept C185592680 @default.
- W2337194523 hasConcept C203014093 @default.
- W2337194523 hasConcept C204232928 @default.
- W2337194523 hasConcept C2776281502 @default.
- W2337194523 hasConcept C2780775167 @default.
- W2337194523 hasConcept C50254741 @default.
- W2337194523 hasConcept C71924100 @default.
- W2337194523 hasConcept C74864618 @default.
- W2337194523 hasConcept C83743174 @default.
- W2337194523 hasConcept C86803240 @default.
- W2337194523 hasConcept C95444343 @default.
- W2337194523 hasConceptScore W2337194523C126322002 @default.
- W2337194523 hasConceptScore W2337194523C134018914 @default.
- W2337194523 hasConceptScore W2337194523C142724271 @default.
- W2337194523 hasConceptScore W2337194523C149614440 @default.
- W2337194523 hasConceptScore W2337194523C149786707 @default.
- W2337194523 hasConceptScore W2337194523C153911025 @default.
- W2337194523 hasConceptScore W2337194523C159654299 @default.
- W2337194523 hasConceptScore W2337194523C169760540 @default.
- W2337194523 hasConceptScore W2337194523C170493617 @default.
- W2337194523 hasConceptScore W2337194523C185592680 @default.
- W2337194523 hasConceptScore W2337194523C203014093 @default.
- W2337194523 hasConceptScore W2337194523C204232928 @default.
- W2337194523 hasConceptScore W2337194523C2776281502 @default.
- W2337194523 hasConceptScore W2337194523C2780775167 @default.
- W2337194523 hasConceptScore W2337194523C50254741 @default.
- W2337194523 hasConceptScore W2337194523C71924100 @default.
- W2337194523 hasConceptScore W2337194523C74864618 @default.
- W2337194523 hasConceptScore W2337194523C83743174 @default.
- W2337194523 hasConceptScore W2337194523C86803240 @default.
- W2337194523 hasConceptScore W2337194523C95444343 @default.
- W2337194523 hasLocation W23371945231 @default.
- W2337194523 hasOpenAccess W2337194523 @default.
- W2337194523 hasPrimaryLocation W23371945231 @default.
- W2337194523 hasRelatedWork W1523218557 @default.
- W2337194523 hasRelatedWork W2001144263 @default.
- W2337194523 hasRelatedWork W2005641411 @default.
- W2337194523 hasRelatedWork W2040390460 @default.
- W2337194523 hasRelatedWork W2040713000 @default.
- W2337194523 hasRelatedWork W2042927086 @default.
- W2337194523 hasRelatedWork W2051815331 @default.
- W2337194523 hasRelatedWork W2053376101 @default.
- W2337194523 hasRelatedWork W2074476845 @default.
- W2337194523 hasRelatedWork W2079228150 @default.
- W2337194523 hasRelatedWork W2091746433 @default.
- W2337194523 hasRelatedWork W2098522772 @default.
- W2337194523 hasRelatedWork W2123349498 @default.
- W2337194523 hasRelatedWork W2130889229 @default.
- W2337194523 hasRelatedWork W2133167865 @default.
- W2337194523 hasRelatedWork W2149706830 @default.
- W2337194523 hasRelatedWork W2155419660 @default.
- W2337194523 hasRelatedWork W2171622847 @default.
- W2337194523 hasRelatedWork W2982466545 @default.
- W2337194523 hasRelatedWork W3013131934 @default.
- W2337194523 isParatext "false" @default.
- W2337194523 isRetracted "false" @default.
- W2337194523 magId "2337194523" @default.
- W2337194523 workType "dissertation" @default.