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- W2337269153 abstract "Somatic embryogenesis and plantlet regeneration from leaf explants of Indian ginseng (Withania somnifera L. Dunal), a medicinally potent plant species is reported. Embryogenic callus was obtained from leaf explants on Murashige and Skoog medium supplemented with 2, 4-dichlorophenoxy acetic acid (0.5-5.0 mg l -1 ) and N 6 -benzylaminopurine (0.5-5.0 mg l -1) . High frequency of somatic embryo mass induction (10.89 ± 0.78) was noticed on 2, 4-D (0.5 mg l -1 ), BAP (1.0 mg l -1 ) along with casein hydrolysate (10.0 mg l -1 ). This nodulated callus upon regular subculturing on the same medium and hormonal regime showed various typical stages of embryo development i.e. heart shaped, torpedo shaped and cotyledonary stages culminating into germination and maturity. Well-developed cotyledonary stage embryos were germinated on MS medium fortified with BAP (0.5 mg l -1 ). Highest percentage of somatic embryo formed shoots on MS medium augmented with BAP (0.5 mg l -1 ), but they did not show germination on MS medium without plant growth regulators. The shoots raised from somatic embryos were rooted on MS medium supplemented with indole-3- butyric acid (1.0 mg l -1 ). The survival rate after transplantation of plantlets was 55%. Plants produced were morphologically similar to mother plants. The present protocol is an important path for genetic transformation studies in Withania somnifera. (Researcher. 2010; 2(3):1-6)." @default.
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- W2337269153 date "2010-01-01" @default.
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- W2337269153 title "PLANT REGENERATION THROUGH IN VITRO SOMATIC EMBRYOGENESIS IN ASHWAGANDHA (Withania somnifera L. Dunal)" @default.
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