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- W2337462079 abstract "α-Hydroxyglutarate dependent NADH oxidizing activity was found in a cell-free extract of Alcaligenes No. 314. Succinate was identified as a product from L-α-hydroxyglutarate in the extract. Stoichiometric conversion of L-α-hydroxyglutarate to succinate occurred under anaerobic conditions without the addition of any cofactors, while the reaction was stimulated by Fe2+. 4,5-Dioxovaleric acid seems to be an intermediate of the reaction since it was converted to succinate and L-α-hydroxyglutarate by the extract. Addition of formaldehyde, another possible product of the succinate forming reaction, to the extract caused oxidation of NADH, which might be responsible for the α-hydroxyglutarate dependent NADH oxidation. Considering the presence of L-α-hydroxyglutarate dehydrogenase in the Alcaligenes cells, an anaerobic by-path of the TCA cycle through which succinate is formed from α-ketoglutarate was proposed." @default.
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- W2337462079 date "1986-05-01" @default.
- W2337462079 modified "2023-10-16" @default.
- W2337462079 title "Metabolism ofα-Hydroxyglutarate by anAlcaligenessp." @default.
- W2337462079 cites W2282054059 @default.
- W2337462079 doi "https://doi.org/10.1080/00021369.1986.10867541" @default.
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