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- W2337462744 abstract "The polymorphism of the Sec2 gene, which determines Se blood type, has been reported. This study presents an Se genotyping system by the allele-specific polymerase chain reaction amplification method. The Se, sej and se fus alleles were amplified using allele-specific primers. The Sec1, Sec2 and se fus genes were analyzed by DNA sequencing. The 299-bp Se, 146bp sej and/or 312-bp se fus allele-specific products were amplified and detected in the native polyacrylamide gel. The 314th‐ 316th nucleotides of the Sec1 gene were CCC, which were different from the nucleotides GGG reported previously by Kelly et al. [J Biol Chem 270 (1995) 4640]. This Se genotyping system is a simple method available for the forensic science field in Japan. The crossover region of the se fus gene is a 164-bp stretch corresponding to the regions between the 253rd and 416th of the" @default.
- W2337462744 created "2016-06-24" @default.
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- W2337462744 date "2002-01-01" @default.
- W2337462744 modified "2023-09-23" @default.
- W2337462744 title "Brief communication Se genotyping following allele-specific polymerase chain reaction amplification" @default.
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