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- W2337508214 abstract "The actomyosin contractile ring is a network of cross-linked actin filaments that facilitates cytokinesis in dividing cells. Contractile ring formation has been well characterized in Schizosaccharomyces pombe, in which the cross-linking protein α-actinin SpAin1 bundles the actin filament network. However, the specific biochemical properties of SpAin1 and whether they are tailored for cytokinesis are not known. Therefore we purified SpAin1 and quantified its ability to dynamically bind and bundle actin filaments in vitro using a combination of bulk sedimentation assays and direct visualization by two-color total internal reflection fluorescence microscopy. We found that, while SpAin1 bundles actin filaments of mixed polarity like other α-actinins, SpAin1 has lower bundling activity and is more dynamic than human α-actinin HsACTN4. To determine whether dynamic bundling is important for cytokinesis in fission yeast, we created the less dynamic bundling mutant SpAin1(R216E). We found that dynamic bundling is critical for cytokinesis, as cells expressing SpAin1(R216E) display disorganized ring material and delays in both ring formation and constriction. Furthermore, computer simulations of initial actin filament elongation and alignment revealed that an intermediate level of cross-linking best facilitates filament alignment. Together our results demonstrate that dynamic bundling by SpAin1 is important for proper contractile ring formation and constriction." @default.
- W2337508214 created "2016-06-24" @default.
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- W2337508214 date "2016-06-01" @default.
- W2337508214 modified "2023-10-17" @default.
- W2337508214 title "The F-actin bundler α-actinin Ain1 is tailored for ring assembly and constriction during cytokinesis in fission yeast" @default.
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- W2337508214 cites W1525011106 @default.
- W2337508214 cites W1538692246 @default.
- W2337508214 cites W1604677470 @default.
- W2337508214 cites W1941858603 @default.
- W2337508214 cites W1972599305 @default.
- W2337508214 cites W1977436020 @default.
- W2337508214 cites W1984559046 @default.
- W2337508214 cites W1985497018 @default.
- W2337508214 cites W1986444740 @default.
- W2337508214 cites W1989108940 @default.
- W2337508214 cites W1993663183 @default.
- W2337508214 cites W1996503565 @default.
- W2337508214 cites W1999143977 @default.
- W2337508214 cites W2000684972 @default.
- W2337508214 cites W2005203145 @default.
- W2337508214 cites W2007719816 @default.
- W2337508214 cites W2010630361 @default.
- W2337508214 cites W2012496083 @default.
- W2337508214 cites W2013570410 @default.
- W2337508214 cites W2027949267 @default.
- W2337508214 cites W2029414348 @default.
- W2337508214 cites W2030388894 @default.
- W2337508214 cites W2033293405 @default.
- W2337508214 cites W2041267262 @default.
- W2337508214 cites W2041578482 @default.
- W2337508214 cites W2041939613 @default.
- W2337508214 cites W2044177027 @default.
- W2337508214 cites W2044363101 @default.
- W2337508214 cites W2046558624 @default.
- W2337508214 cites W2047369914 @default.
- W2337508214 cites W2050301961 @default.
- W2337508214 cites W2050612706 @default.
- W2337508214 cites W2051501508 @default.
- W2337508214 cites W2052985914 @default.
- W2337508214 cites W2054225562 @default.
- W2337508214 cites W2056462932 @default.
- W2337508214 cites W2056889742 @default.
- W2337508214 cites W2058377873 @default.
- W2337508214 cites W2059943461 @default.
- W2337508214 cites W2067841569 @default.
- W2337508214 cites W2074599069 @default.
- W2337508214 cites W2075966687 @default.
- W2337508214 cites W2079149769 @default.
- W2337508214 cites W2079161230 @default.
- W2337508214 cites W2082848316 @default.
- W2337508214 cites W2083215599 @default.
- W2337508214 cites W2083523423 @default.
- W2337508214 cites W2084555208 @default.
- W2337508214 cites W2085308794 @default.
- W2337508214 cites W2085496398 @default.
- W2337508214 cites W2089155140 @default.
- W2337508214 cites W2090736109 @default.
- W2337508214 cites W2091289978 @default.
- W2337508214 cites W2091950241 @default.
- W2337508214 cites W2103302030 @default.
- W2337508214 cites W2105402224 @default.
- W2337508214 cites W2106061122 @default.
- W2337508214 cites W2108167818 @default.
- W2337508214 cites W2115836137 @default.
- W2337508214 cites W2117494028 @default.
- W2337508214 cites W2118272396 @default.
- W2337508214 cites W2119836020 @default.
- W2337508214 cites W2121734455 @default.
- W2337508214 cites W2121915052 @default.
- W2337508214 cites W2123735680 @default.
- W2337508214 cites W2127303707 @default.
- W2337508214 cites W2129037338 @default.
- W2337508214 cites W2131429362 @default.
- W2337508214 cites W2136845571 @default.
- W2337508214 cites W2138618719 @default.
- W2337508214 cites W2140706483 @default.
- W2337508214 cites W2149909679 @default.
- W2337508214 cites W2165091194 @default.
- W2337508214 cites W2319797259 @default.
- W2337508214 cites W2322219433 @default.
- W2337508214 cites W2412234989 @default.
- W2337508214 cites W84272969 @default.
- W2337508214 doi "https://doi.org/10.1091/mbc.e16-01-0010" @default.
- W2337508214 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4884072" @default.
- W2337508214 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27075176" @default.
- W2337508214 hasPublicationYear "2016" @default.
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