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• W2337685174 abstract "Objective and design The aim of this study was to investigate the effect of heat shock protein 70 (HSP70) on the mRNA expression of tumor necrosis factor-alpha (TNF-a) and receptor activator of nuclear factor-kappa B ligand (RANKL) induced by compressive forces (CF) in human periodontal ligament (hPDL) cells. Material and treatment hPDL cells were subjected to 1.0, 2.0, or 4.0 g/cm 2 of CF for 24 h, and were treated with recombinant human inducible HSP70 for 12 h. Methods The mRNA expression of HSP27, HSP70, HSP90, TNF-a, RANKL and OPG from hPDL cells subjected to CF was determined by real-time PCR. The protein production of HSP70 was determined by Western blot analysis and ELISA. Results The mRNA expression of HSP70, TNF-a and RANKL were found to be increased in a time- and magnitude-dependent manner, detectable at 12, 9, and 9 h, respectively. TNF-a and RANKL expression gradually decreased at 12 h with increasing HSP70 levels, and further decreased thereafter. Furthermore, exogenous HSP70 partially inhibited the CF-induced TNF-a and RANKL expression in a dose-dependent manner at 6 and 12 h. Conclusions These results indicate that HSP70 may modulate the mRNA expression of TNF-a and RANKL in hPDL cells in response to CF." @default.
• W2337685174 created "2016-06-24" @default.
• W2337685174 creator A5064522694 @default.
• W2337685174 date "2011-01-01" @default.
• W2337685174 modified "2023-09-24" @default.
• W2337685174 title "Effects of HSP70 on the compression force-induced TNF-a and RANKL expression in human periodontal ligament cells" @default.
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