FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2337722812> ?p ?o ?g. }

• W2337722812 endingPage "455" @default.
• W2337722812 startingPage "446" @default.
• W2337722812 abstract "Biological heart valve prostheses are characterized by a limited durability due to the degenerative processes after implantation. Tissue engineering may provide new approaches in the development of optimized valvular grafts. While re-endothelialization of decellularized heart valves has already been successfully implemented, interstitial repopulation still remains an unaccomplished objective although it is essential for valvular functionality and regeneration potential. The aim of this study was to compare different concepts for an improved in vitro interstitial repopulation of decellularized heart valves. A novel 3D heart valve model has been developed to investigate the cell behaviour of valvular interstitial cells (VIC) in their physiological environment and to evaluate the potential of in vitro repopulation of acellular heart valves.Ovine aortic heart valves were decellularized by detergent solutions and additionally treated with trypsin or laser perforation. Subsequently, the decellularized extracellular matrices (dECM) were reseeded with ovine VIC using reseeding devices to provide a repopulation of the matrix on a defined area under controlled conditions. After an initial attachment of the VIC, reseeded dECM were transferred into a transwell system to improve the nutrient supply inside the valvular matrix. Cell migration and expression of cell markers were analysed histologically. The results were compared with VIC cultivation in a biological scaffold.VIC did not migrate into the matrix of untreated dECM and reseeding in laser perforated dECM showed inconsistent results. However, trypsinization increased the susceptibility of the valvular cusps to VIC penetration and repopulation of superficial areas. Additionally, the cultivation of reseeded dECM in a transwell system significantly increased the total number of cells repopulating the valvular matrix and their mean migration distance, representing the best repopulation results. Immunohistological analysis and in situ zymography revealed a low activation status of repopulating VIC after 7 days of culture.A comparison of different techniques for increasing interstitial repopulation of detergent dECM revealed that an additional limited trypsin treatment was most effective. Nevertheless, a complete interstitial repopulation of decellularized heart valves remains a challenging endeavour. Additional experimental fine-tuning may improve the in vitro results of heart valve tissue engineering." @default.
• W2337722812 created "2016-06-24" @default.
• W2337722812 creator A5019986996 @default.
• W2337722812 creator A5044948875 @default.
• W2337722812 creator A5054596824 @default.
• W2337722812 creator A5061184512 @default.
• W2337722812 creator A5063048328 @default.
• W2337722812 creator A5067547175 @default.
• W2337722812 creator A5067636326 @default.
• W2337722812 creator A5084075182 @default.
• W2337722812 creator A5091872212 @default.
• W2337722812 date "2016-04-15" @default.
• W2337722812 modified "2023-09-26" @default.
• W2337722812 title "Challenges in developing a reseeded, tissue-engineered aortic valve prosthesis" @default.
• W2337722812 cites W1581866053 @default.
• W2337722812 cites W1970564951 @default.
• W2337722812 cites W1984215221 @default.
• W2337722812 cites W1996627890 @default.
• W2337722812 cites W2000464667 @default.
• W2337722812 cites W2006673751 @default.
• W2337722812 cites W2016028892 @default.
• W2337722812 cites W2032381326 @default.
• W2337722812 cites W2046385728 @default.
• W2337722812 cites W2059385371 @default.
• W2337722812 cites W2077000402 @default.
• W2337722812 cites W2109157083 @default.
• W2337722812 cites W2111969582 @default.
• W2337722812 cites W2115740294 @default.
• W2337722812 cites W2124719174 @default.
• W2337722812 cites W2144226542 @default.
• W2337722812 cites W2144740404 @default.
• W2337722812 cites W2158927007 @default.
• W2337722812 cites W2171313781 @default.
• W2337722812 cites W3098551216 @default.
• W2337722812 cites W4293084779 @default.
• W2337722812 doi "https://doi.org/10.1093/ejcts/ezw057" @default.
• W2337722812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27084195" @default.
• W2337722812 hasPublicationYear "2016" @default.
• W2337722812 type Work @default.
• W2337722812 sameAs 2337722812 @default.
• W2337722812 citedByCount "9" @default.
• W2337722812 countsByYear W23377228122017 @default.
• W2337722812 countsByYear W23377228122018 @default.
• W2337722812 countsByYear W23377228122019 @default.
• W2337722812 countsByYear W23377228122020 @default.
• W2337722812 countsByYear W23377228122022 @default.
• W2337722812 crossrefType "journal-article" @default.
• W2337722812 hasAuthorship W2337722812A5019986996 @default.
• W2337722812 hasAuthorship W2337722812A5044948875 @default.
• W2337722812 hasAuthorship W2337722812A5054596824 @default.
• W2337722812 hasAuthorship W2337722812A5061184512 @default.
• W2337722812 hasAuthorship W2337722812A5063048328 @default.
• W2337722812 hasAuthorship W2337722812A5067547175 @default.
• W2337722812 hasAuthorship W2337722812A5067636326 @default.
• W2337722812 hasAuthorship W2337722812A5084075182 @default.
• W2337722812 hasAuthorship W2337722812A5091872212 @default.
• W2337722812 hasBestOaLocation W23377228121 @default.
• W2337722812 hasConcept C105702510 @default.
• W2337722812 hasConcept C109159458 @default.
• W2337722812 hasConcept C136229726 @default.
• W2337722812 hasConcept C164705383 @default.
• W2337722812 hasConcept C171056886 @default.
• W2337722812 hasConcept C185592680 @default.
• W2337722812 hasConcept C189165786 @default.
• W2337722812 hasConcept C190672674 @default.
• W2337722812 hasConcept C2776229121 @default.
• W2337722812 hasConcept C2780918802 @default.
• W2337722812 hasConcept C28328180 @default.
• W2337722812 hasConcept C49892992 @default.
• W2337722812 hasConcept C71924100 @default.
• W2337722812 hasConcept C86803240 @default.
• W2337722812 hasConcept C95444343 @default.
• W2337722812 hasConceptScore W2337722812C105702510 @default.
• W2337722812 hasConceptScore W2337722812C109159458 @default.
• W2337722812 hasConceptScore W2337722812C136229726 @default.
• W2337722812 hasConceptScore W2337722812C164705383 @default.
• W2337722812 hasConceptScore W2337722812C171056886 @default.
• W2337722812 hasConceptScore W2337722812C185592680 @default.
• W2337722812 hasConceptScore W2337722812C189165786 @default.
• W2337722812 hasConceptScore W2337722812C190672674 @default.
• W2337722812 hasConceptScore W2337722812C2776229121 @default.
• W2337722812 hasConceptScore W2337722812C2780918802 @default.
• W2337722812 hasConceptScore W2337722812C28328180 @default.
• W2337722812 hasConceptScore W2337722812C49892992 @default.
• W2337722812 hasConceptScore W2337722812C71924100 @default.
• W2337722812 hasConceptScore W2337722812C86803240 @default.
• W2337722812 hasConceptScore W2337722812C95444343 @default.
• W2337722812 hasIssue "3" @default.
• W2337722812 hasLocation W23377228121 @default.
• W2337722812 hasLocation W23377228122 @default.
• W2337722812 hasOpenAccess W2337722812 @default.
• W2337722812 hasPrimaryLocation W23377228121 @default.
• W2337722812 hasRelatedWork W1489387847 @default.
• W2337722812 hasRelatedWork W1985597509 @default.
• W2337722812 hasRelatedWork W2069097422 @default.
• W2337722812 hasRelatedWork W2075935312 @default.
• W2337722812 hasRelatedWork W2092144208 @default.
• W2337722812 hasRelatedWork W2155532705 @default.

• next