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- W2337755856 abstract "The ribosomal internal transcribed spacer (ITS) region from individual Gyrodactylus specimens was amplified by polymerase chain reaction (PCR). The reaction amplified the entire ITS -5.8S-ITS2 region of the ribosomal RNA gene cluster using primers that hybridize to the 3' terminus of the small subunit and the 5' terminus of the large subunit ribosomal RNA genes. The PCR products from Gyrodactylus salaris and Gyrodactylus thymalli were cloned and sequenced. The Gyrodactylus 5.8S gene was identified following comparative alignment of the G. salaris sequence and a Schistosoma 5.8S gene sequence. The ITS regions from G. salaris, G. thymalli, Gyrodactylus derjavini, and Gyrodactylus truttae were compared by restriction enzyme analysis and interspecific restriction fragment length polymorphisms were found. Gyrodactylus salaris and G. thymalli restriction fragment sizes were confirmed from sequence data. ITS amplification followed by Sau3AI digestion enables rapid and clear differentiation of G. salaris, G. derjavini, and G. truttae. More than 400 species of Gyrodactylus have been described. Subgenera have been defined on the basis of the morphology of the excretory system and each subgenus can be divided into species groups based on opisthaptor morphology (Malmberg, 1970). Intensive study of 1 species, Gyrodactylus salaris Malm- berg, 1957, has shown that factors such as temperature, host, and geographical location can result in variation in opisthaptor morphology (Ergens, 1981; Mo, 1991a, 1991b, 1991c, 1993). This can make species identification difficult, as can the occur- rence of mixed infections of more than 1 Gyrodactylus species in a single host (Malmberg, 1964; Cone et al., 1983). Gyrodactylus salaris infections have had a catastrophic effect on wild Atlantic salmon (Salmo salar L.) stocks in Norway (Mo, 1994). To prevent the spread of this pathogen to other areas or countries, objective methods of Gyrodactylus species identification are required. Study of the molecular genetics of gyrodactylids has been undertaken to develop such methods and has concentrated on the ribosomal RNA (rRNA) genes." @default.
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- W2337755856 date "1997-01-01" @default.
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- W2337755856 title "SPECIES VARIATION WITHIN THE INTERNAL TRANSCRIBED SPACER (ITS) REGION OF GYRODACTYLUS (MONOGENEA: GYRODACTYLIDAE)" @default.
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