Matches in SemOpenAlex for { <https://semopenalex.org/work/W2337853668> ?p ?o ?g. }
- W2337853668 abstract "Chronic infection by high risk human papillomavirus (HPV) strains may lead to cancer. Expression of the two viral oncoproteins E6 and E7 is largely responsible for immortalization of infected cells. The HPV E6 is a small (approximately 150 residues) two domain protein that interacts with a number of cellular proteins including the ubiquitin ligase E6-associated protein (E6AP) and several PDZ-domain containing proteins. Our aim was to design a high-affinity binder for HPV E6 by linking two of its cellular targets. First, we improved the affinity of the second PDZ domain from SAP97 for the C-terminus of HPV E6 from the high-risk strain HPV18 using phage display. Second, we added a helix from E6AP to the N-terminus of the optimized PDZ variant, creating a chimeric bivalent binder, denoted PDZbody. Full-length HPV E6 proteins are difficult to express and purify. Nevertheless, we could measure the affinity of the PDZbody for E6 from another high-risk strain, HPV16 (Kd 565 nM). Finally, the PDZbody was used to co-immunoprecipitate E6 protein from HPV18-immortalized HeLa cells, confirming the interaction between PDZbody and HPV18 E6 in a cellular context." @default.
- W2337853668 created "2016-06-24" @default.
- W2337853668 creator A5001315246 @default.
- W2337853668 creator A5014610427 @default.
- W2337853668 creator A5020398273 @default.
- W2337853668 creator A5031768698 @default.
- W2337853668 creator A5045635957 @default.
- W2337853668 creator A5060277257 @default.
- W2337853668 creator A5067743846 @default.
- W2337853668 creator A5078066253 @default.
- W2337853668 date "2015-01-01" @default.
- W2337853668 modified "2023-09-24" @default.
- W2337853668 title "Design of a PDZbody, a bivalent binder of the E6 protein from human" @default.
- W2337853668 cites W1946074662 @default.
- W2337853668 cites W1964736119 @default.
- W2337853668 cites W1979715740 @default.
- W2337853668 cites W1987464084 @default.
- W2337853668 cites W1990350046 @default.
- W2337853668 cites W1991509069 @default.
- W2337853668 cites W1992974270 @default.
- W2337853668 cites W1992976870 @default.
- W2337853668 cites W1993568759 @default.
- W2337853668 cites W1994353590 @default.
- W2337853668 cites W1995605583 @default.
- W2337853668 cites W1997938520 @default.
- W2337853668 cites W2005747738 @default.
- W2337853668 cites W2006786639 @default.
- W2337853668 cites W2009503206 @default.
- W2337853668 cites W2016430335 @default.
- W2337853668 cites W2020512831 @default.
- W2337853668 cites W2022403117 @default.
- W2337853668 cites W2022747364 @default.
- W2337853668 cites W2034496567 @default.
- W2337853668 cites W2039430313 @default.
- W2337853668 cites W2043189903 @default.
- W2337853668 cites W2045050273 @default.
- W2337853668 cites W2052323707 @default.
- W2337853668 cites W2053489904 @default.
- W2337853668 cites W2056169996 @default.
- W2337853668 cites W2060786724 @default.
- W2337853668 cites W2062808707 @default.
- W2337853668 cites W2066332001 @default.
- W2337853668 cites W2067060164 @default.
- W2337853668 cites W2068965703 @default.
- W2337853668 cites W2077239477 @default.
- W2337853668 cites W2084938664 @default.
- W2337853668 cites W2087549969 @default.
- W2337853668 cites W2101653819 @default.
- W2337853668 cites W2108279669 @default.
- W2337853668 cites W2121046447 @default.
- W2337853668 cites W2124838000 @default.
- W2337853668 cites W2137790471 @default.
- W2337853668 cites W2142114240 @default.
- W2337853668 cites W2143282087 @default.
- W2337853668 cites W2144553406 @default.
- W2337853668 cites W2145457496 @default.
- W2337853668 cites W2145902150 @default.
- W2337853668 cites W2154719936 @default.
- W2337853668 cites W2157826046 @default.
- W2337853668 cites W2163872654 @default.
- W2337853668 cites W2167415438 @default.
- W2337853668 cites W2170939558 @default.
- W2337853668 cites W2405345539 @default.
- W2337853668 hasPublicationYear "2015" @default.
- W2337853668 type Work @default.
- W2337853668 sameAs 2337853668 @default.
- W2337853668 citedByCount "0" @default.
- W2337853668 crossrefType "journal-article" @default.
- W2337853668 hasAuthorship W2337853668A5001315246 @default.
- W2337853668 hasAuthorship W2337853668A5014610427 @default.
- W2337853668 hasAuthorship W2337853668A5020398273 @default.
- W2337853668 hasAuthorship W2337853668A5031768698 @default.
- W2337853668 hasAuthorship W2337853668A5045635957 @default.
- W2337853668 hasAuthorship W2337853668A5060277257 @default.
- W2337853668 hasAuthorship W2337853668A5067743846 @default.
- W2337853668 hasAuthorship W2337853668A5078066253 @default.
- W2337853668 hasConcept C104317684 @default.
- W2337853668 hasConcept C123894998 @default.
- W2337853668 hasConcept C126322002 @default.
- W2337853668 hasConcept C134459356 @default.
- W2337853668 hasConcept C153911025 @default.
- W2337853668 hasConcept C154428179 @default.
- W2337853668 hasConcept C178790620 @default.
- W2337853668 hasConcept C185592680 @default.
- W2337853668 hasConcept C202751555 @default.
- W2337853668 hasConcept C25602115 @default.
- W2337853668 hasConcept C2776620830 @default.
- W2337853668 hasConcept C2777366897 @default.
- W2337853668 hasConcept C2994532551 @default.
- W2337853668 hasConcept C40767141 @default.
- W2337853668 hasConcept C544153396 @default.
- W2337853668 hasConcept C55493867 @default.
- W2337853668 hasConcept C71924100 @default.
- W2337853668 hasConcept C86803240 @default.
- W2337853668 hasConcept C95444343 @default.
- W2337853668 hasConceptScore W2337853668C104317684 @default.
- W2337853668 hasConceptScore W2337853668C123894998 @default.
- W2337853668 hasConceptScore W2337853668C126322002 @default.
- W2337853668 hasConceptScore W2337853668C134459356 @default.
- W2337853668 hasConceptScore W2337853668C153911025 @default.