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- W2338040041 abstract "ABSTRACTPolarity proteins cdc24 and bem1 regulate polar growth, cytoskeleton morphogenesis, and the generation of reactive oxygen species (ROS) in filamentous fungi. In this study, the Mrcdc24 and Mrbem1 genes were cloned from Metarhizium rileyi and found to encode 1006 and 562 amino acid proteins, respectively. To determine the functions of Mrcdc24 and Mrbem1, gene-silencing mutants Mrcdc24RNAi, Mrbem1RNAi, and Mrcdc24&Nrbem1RNAi were generated by RNA silencing. The mutants presented significant phenotype changes in hyphal growth, and conidial yields, microsclerotia yields and virulence were reduced. Furthermore, the transcription levels of the MrnoxA and MrnoxR, which regulate ROS generation in the Mrcdc24RNAi and Mrbem1RNAi strains, were reduced. The transcriptional levels of MrracA and Mrcdc42 were significantly reduced in the Mrcdc24RNAi strains. Our results confirmed the crucial role of genes Mrcdc24 and Mrbem1 in hyphal growth, conidiation, microsclerotia formation, ROS generation and virulence." @default.
- W2338040041 created "2016-06-24" @default.
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- W2338040041 date "2016-04-13" @default.
- W2338040041 modified "2023-09-22" @default.
- W2338040041 title "Polarity proteins Mrcdc24 and Mrbem1 required for hypha growth and microsclerotia formation inMetarhizium rileyi" @default.
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- W2338040041 doi "https://doi.org/10.1080/09583157.2015.1120274" @default.
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