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- W2338382502 abstract "Endothelial microparticles (EMPs) are complex structures with pleiotropic properties and are emerging as an index of endothelial damage. Previous studies have shown elevated circulating EMP levels in inflammatory disorders, which raises the question about their physiological role. This study aims to investigate the autocrine effects of EMPs on TNFα-mediated EMP release, as well as on endothelial cell activation and migration. Two groups of EMPs were generated in vitro from TNF-stimulated human umbilical vein endothelial cells (HUVECs; sEMPs) or untreated HUVECs (uEMPs) and collected by ultracentrifugation. HUVECs were incubated with either 106 sEMPs or uEMPs/ml in the presence or absence of TNFα (10 ng/ml) for 24 h. EMP levels were enumerated by flow cytometry; RT-PCR and functional scratch assays were also carried out. HUVECs treated with either sEMP or uEMP showed increased ICAM-1 mRNA abundance (p" @default.
- W2338382502 created "2016-06-24" @default.
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- W2338382502 date "2015-11-01" @default.
- W2338382502 modified "2023-09-23" @default.
- W2338382502 title "36 Endothelial microparticles: investigating their role on endothelial cells in vitro" @default.
- W2338382502 doi "https://doi.org/10.1136/heartjnl-2015-308734.36" @default.
- W2338382502 hasPublicationYear "2015" @default.
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