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• W2338628486 abstract "Polyhydroxyalkanoates (PHAs) containing hydroxyl groups in a side chain were produced in recombinant Escherichia coli JM109 using glycolate as the sole carbon source. The propionate-CoA transferase (pct) gene from Megasphaera elsdenii and the β-ketothiolase (bktB) gene and phaCAB operon from Ralstonia eutropha H16 were introduced into E. coli JM109. A novel monomer containing a hydroxyl group, dihydroxybutyrate (DHBA), was the expected product of the condensation of glycolyl-CoA and acetyl-CoA by BktB. The recombinant strain produced a PHA containing 1 mol% DHBA. The incorporation of DHBA may have been restricted because the expression of phaAB1 competes for acetyl-CoA. The PHA containing DHBA units were evaluated regarding thermal properties, such as melting temperature, glass transition temperature and thermal degradation temperature. The current study demonstrates a potential use of PHA containing hydroxyl groups as renewable resources in biological materials." @default.
• W2338628486 created "2016-06-24" @default.
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• W2338628486 date "2016-04-14" @default.
• W2338628486 modified "2023-10-14" @default.
• W2338628486 title "Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli" @default.
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• W2338628486 doi "https://doi.org/10.1186/s13568-016-0200-5" @default.
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