FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2338647568> ?p ?o ?g. }

• W2338647568 endingPage "118" @default.
• W2338647568 startingPage "110" @default.
• W2338647568 abstract "Human gingival fibroblasts (hGFs) present an attractive source of induced pluripotent stem cells (iPSCs), which are expected to be a powerful tool for regenerative dentistry. However, problems to be addressed prior to clinical application include the use of animal-derived feeder cells for cultures. The aim of this study was to establish an autologous hGF-derived iPSC (hGF-iPSC) culture system by evaluating the feeder ability of hGFs. In both serum-containing and serum-free media, hGFs showed higher proliferation than human dermal fibroblasts (hDFs). Three hGF strains were isolated under serum-free conditions, although 2 showed impaired proliferation. When hGF-iPSCs were transferred onto mitomycin C-inactivated hGFs, hDFs, or mouse-derived SNL feeders, hGF and SNL feeders were clearly hGF-iPSC supportive for more than 50 passages, whereas hDF feeders were only able to maintain undifferentiated hGF-iPSC growth for a few passages. After 20 passages on hGF feeders, embryonic stem cell marker expression and CpG methylation at the NANOG and OCT3/4 promoters were similar for hGF-iPSCs cultured on hGF and SNL feeder cells. Long-term cultures of hGF-iPSCs on hGF feeders sustained their normal karyotype and pluripotency. On hGF feeders, hGF-iPSC colonies were surrounded by many colony-derived fibroblast-like cells, and the size of intact colonies at 7 d after passage was significantly larger than that on SNL feeders. Allogeneic hGF strains also maintained hGF-iPSCs for 10 passages. Compared with hDFs, hGFs showed a higher production of laminin-332, laminin α5 chain, and insulin-like growth factor-II, which have been reported to sustain the long-term self-renewal of pluripotent stem cells. These results suggest that hGFs possess an excellent feeder capability and thus can be used as alternatives to conventional mouse-derived SNL and hDF feeders. In addition, our findings suggest that hGF feeders are promising candidates for animal component-free ex vivo expansion of autologous hGF-iPSCs, thus providing an important step toward the future therapeutic application of hGF-iPSCs." @default.
• W2338647568 created "2016-06-24" @default.
• W2338647568 creator A5030551724 @default.
• W2338647568 creator A5038525852 @default.
• W2338647568 creator A5040162933 @default.
• W2338647568 creator A5041949930 @default.
• W2338647568 creator A5053775658 @default.
• W2338647568 creator A5066111166 @default.
• W2338647568 creator A5076006520 @default.
• W2338647568 creator A5080030836 @default.
• W2338647568 date "2015-10-14" @default.
• W2338647568 modified "2023-09-27" @default.
• W2338647568 title "Gingival Fibroblasts as Autologous Feeders for Induced Pluripotent Stem Cells" @default.
• W2338647568 cites W126028056 @default.
• W2338647568 cites W1860109924 @default.
• W2338647568 cites W1969544384 @default.
• W2338647568 cites W1986297657 @default.
• W2338647568 cites W1988634174 @default.
• W2338647568 cites W1990110893 @default.
• W2338647568 cites W1995438382 @default.
• W2338647568 cites W1996489883 @default.
• W2338647568 cites W1998141644 @default.
• W2338647568 cites W2002163959 @default.
• W2338647568 cites W2016283026 @default.
• W2338647568 cites W2030917251 @default.
• W2338647568 cites W2036829726 @default.
• W2338647568 cites W2040502563 @default.
• W2338647568 cites W2040671717 @default.
• W2338647568 cites W2054210108 @default.
• W2338647568 cites W2070677575 @default.
• W2338647568 cites W2072621038 @default.
• W2338647568 cites W2075655507 @default.
• W2338647568 cites W2076439120 @default.
• W2338647568 cites W2082292567 @default.
• W2338647568 cites W2089747751 @default.
• W2338647568 cites W2106774689 @default.
• W2338647568 cites W2121798607 @default.
• W2338647568 cites W2125987139 @default.
• W2338647568 cites W2131162243 @default.
• W2338647568 cites W2142308374 @default.
• W2338647568 cites W2144345918 @default.
• W2338647568 cites W2144396367 @default.
• W2338647568 cites W2159985165 @default.
• W2338647568 cites W2175657468 @default.
• W2338647568 cites W653281146 @default.
• W2338647568 doi "https://doi.org/10.1177/0022034515611602" @default.
• W2338647568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26467419" @default.
• W2338647568 hasPublicationYear "2015" @default.
• W2338647568 type Work @default.
• W2338647568 sameAs 2338647568 @default.
• W2338647568 citedByCount "14" @default.
• W2338647568 countsByYear W23386475682016 @default.
• W2338647568 countsByYear W23386475682017 @default.
• W2338647568 countsByYear W23386475682018 @default.
• W2338647568 countsByYear W23386475682019 @default.
• W2338647568 countsByYear W23386475682020 @default.
• W2338647568 countsByYear W23386475682021 @default.
• W2338647568 countsByYear W23386475682023 @default.
• W2338647568 crossrefType "journal-article" @default.
• W2338647568 hasAuthorship W2338647568A5030551724 @default.
• W2338647568 hasAuthorship W2338647568A5038525852 @default.
• W2338647568 hasAuthorship W2338647568A5040162933 @default.
• W2338647568 hasAuthorship W2338647568A5041949930 @default.
• W2338647568 hasAuthorship W2338647568A5053775658 @default.
• W2338647568 hasAuthorship W2338647568A5066111166 @default.
• W2338647568 hasAuthorship W2338647568A5076006520 @default.
• W2338647568 hasAuthorship W2338647568A5080030836 @default.
• W2338647568 hasConcept C104317684 @default.
• W2338647568 hasConcept C107459253 @default.
• W2338647568 hasConcept C145103041 @default.
• W2338647568 hasConcept C170493617 @default.
• W2338647568 hasConcept C2776996007 @default.
• W2338647568 hasConcept C2778265714 @default.
• W2338647568 hasConcept C28328180 @default.
• W2338647568 hasConcept C54355233 @default.
• W2338647568 hasConcept C55493867 @default.
• W2338647568 hasConcept C81885089 @default.
• W2338647568 hasConcept C86803240 @default.
• W2338647568 hasConcept C95444343 @default.
• W2338647568 hasConceptScore W2338647568C104317684 @default.
• W2338647568 hasConceptScore W2338647568C107459253 @default.
• W2338647568 hasConceptScore W2338647568C145103041 @default.
• W2338647568 hasConceptScore W2338647568C170493617 @default.
• W2338647568 hasConceptScore W2338647568C2776996007 @default.
• W2338647568 hasConceptScore W2338647568C2778265714 @default.
• W2338647568 hasConceptScore W2338647568C28328180 @default.
• W2338647568 hasConceptScore W2338647568C54355233 @default.
• W2338647568 hasConceptScore W2338647568C55493867 @default.
• W2338647568 hasConceptScore W2338647568C81885089 @default.
• W2338647568 hasConceptScore W2338647568C86803240 @default.
• W2338647568 hasConceptScore W2338647568C95444343 @default.
• W2338647568 hasIssue "1" @default.
• W2338647568 hasLocation W23386475681 @default.
• W2338647568 hasLocation W23386475682 @default.
• W2338647568 hasOpenAccess W2338647568 @default.
• W2338647568 hasPrimaryLocation W23386475681 @default.
• W2338647568 hasRelatedWork W2009859786 @default.
• W2338647568 hasRelatedWork W2017217582 @default.

• next