Matches in SemOpenAlex for { <https://semopenalex.org/work/W2338693143> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2338693143 endingPage "1536" @default.
- W2338693143 startingPage "1532" @default.
- W2338693143 abstract "Translation regulation is a fundamental component of gene expression, allowing cells to respond rapidly to a variety of stimuli in the absence of new transcription. The lack of methods for profiling nascent proteomes in distinct cell populations in heterogeneous tissues has precluded an understanding of translational regulation in physiologically relevant contexts. Here, we describe a chemical genetic method that involves orthogonal enzyme-mediated incorporation of a clickable puromycin analogue into nascent polypeptides. Using this method, we show that we can label newly synthesized proteins in a cell-specific manner in cells grown in culture and in heterogeneous tissues. We also show that we can identify the nascent proteome in genetically targeted cell populations using affinity enrichment and tandem mass spectrometry. Our method has the potential to provide unprecedented insights into cell-specific translational regulation in heterogeneous tissues." @default.
- W2338693143 created "2016-06-24" @default.
- W2338693143 creator A5019803563 @default.
- W2338693143 creator A5030992409 @default.
- W2338693143 creator A5031535970 @default.
- W2338693143 creator A5044379193 @default.
- W2338693143 creator A5090535134 @default.
- W2338693143 date "2016-04-21" @default.
- W2338693143 modified "2023-10-17" @default.
- W2338693143 title "Cell-specific Profiling of Nascent Proteomes Using Orthogonal Enzyme-mediated Puromycin Incorporation" @default.
- W2338693143 cites W1965990486 @default.
- W2338693143 cites W1966901093 @default.
- W2338693143 cites W1992502609 @default.
- W2338693143 cites W1994709846 @default.
- W2338693143 cites W2048105864 @default.
- W2338693143 cites W2070103781 @default.
- W2338693143 cites W2074056212 @default.
- W2338693143 cites W2079838847 @default.
- W2338693143 cites W2094646458 @default.
- W2338693143 cites W2110062500 @default.
- W2338693143 cites W2127056518 @default.
- W2338693143 cites W2132051882 @default.
- W2338693143 cites W2150626351 @default.
- W2338693143 cites W2155925913 @default.
- W2338693143 cites W2159317228 @default.
- W2338693143 cites W2174804804 @default.
- W2338693143 cites W4211097858 @default.
- W2338693143 cites W2050222321 @default.
- W2338693143 doi "https://doi.org/10.1021/acschembio.5b01076" @default.
- W2338693143 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27074634" @default.
- W2338693143 hasPublicationYear "2016" @default.
- W2338693143 type Work @default.
- W2338693143 sameAs 2338693143 @default.
- W2338693143 citedByCount "37" @default.
- W2338693143 countsByYear W23386931432017 @default.
- W2338693143 countsByYear W23386931432018 @default.
- W2338693143 countsByYear W23386931432019 @default.
- W2338693143 countsByYear W23386931432020 @default.
- W2338693143 countsByYear W23386931432021 @default.
- W2338693143 countsByYear W23386931432022 @default.
- W2338693143 countsByYear W23386931432023 @default.
- W2338693143 crossrefType "journal-article" @default.
- W2338693143 hasAuthorship W2338693143A5019803563 @default.
- W2338693143 hasAuthorship W2338693143A5030992409 @default.
- W2338693143 hasAuthorship W2338693143A5031535970 @default.
- W2338693143 hasAuthorship W2338693143A5044379193 @default.
- W2338693143 hasAuthorship W2338693143A5090535134 @default.
- W2338693143 hasConcept C104317684 @default.
- W2338693143 hasConcept C104397665 @default.
- W2338693143 hasConcept C105580179 @default.
- W2338693143 hasConcept C1491633281 @default.
- W2338693143 hasConcept C149364088 @default.
- W2338693143 hasConcept C150194340 @default.
- W2338693143 hasConcept C18431079 @default.
- W2338693143 hasConcept C2779200571 @default.
- W2338693143 hasConcept C3675279 @default.
- W2338693143 hasConcept C55493867 @default.
- W2338693143 hasConcept C70721500 @default.
- W2338693143 hasConcept C86803240 @default.
- W2338693143 hasConcept C95444343 @default.
- W2338693143 hasConceptScore W2338693143C104317684 @default.
- W2338693143 hasConceptScore W2338693143C104397665 @default.
- W2338693143 hasConceptScore W2338693143C105580179 @default.
- W2338693143 hasConceptScore W2338693143C1491633281 @default.
- W2338693143 hasConceptScore W2338693143C149364088 @default.
- W2338693143 hasConceptScore W2338693143C150194340 @default.
- W2338693143 hasConceptScore W2338693143C18431079 @default.
- W2338693143 hasConceptScore W2338693143C2779200571 @default.
- W2338693143 hasConceptScore W2338693143C3675279 @default.
- W2338693143 hasConceptScore W2338693143C55493867 @default.
- W2338693143 hasConceptScore W2338693143C70721500 @default.
- W2338693143 hasConceptScore W2338693143C86803240 @default.
- W2338693143 hasConceptScore W2338693143C95444343 @default.
- W2338693143 hasFunder F4320309233 @default.
- W2338693143 hasFunder F4320310465 @default.
- W2338693143 hasFunder F4320337346 @default.
- W2338693143 hasIssue "6" @default.
- W2338693143 hasLocation W23386931431 @default.
- W2338693143 hasLocation W23386931432 @default.
- W2338693143 hasOpenAccess W2338693143 @default.
- W2338693143 hasPrimaryLocation W23386931431 @default.
- W2338693143 hasRelatedWork W1968284764 @default.
- W2338693143 hasRelatedWork W2009966535 @default.
- W2338693143 hasRelatedWork W2052655934 @default.
- W2338693143 hasRelatedWork W2159317228 @default.
- W2338693143 hasRelatedWork W2168765510 @default.
- W2338693143 hasRelatedWork W22642915 @default.
- W2338693143 hasRelatedWork W2313211238 @default.
- W2338693143 hasRelatedWork W2895314412 @default.
- W2338693143 hasRelatedWork W3036379209 @default.
- W2338693143 hasRelatedWork W3080614974 @default.
- W2338693143 hasVolume "11" @default.
- W2338693143 isParatext "false" @default.
- W2338693143 isRetracted "false" @default.
- W2338693143 magId "2338693143" @default.
- W2338693143 workType "article" @default.