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• W2338695783 abstract "The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. Here, we show the ability to express ssDNAs in Escherichia coli (32-205 nt), which can form structures in vivo or be purified for in vitro assembly. Each ssDNA is encoded by a gene that is transcribed into non-coding RNA containing a 3'-hairpin (HTBS). HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. Purified ssDNA that is produced in vivo is used to assemble large 1D wires (300 nm) and 2D sheets (5.8 μm(2)) in vitro. Intracellular assembly is demonstrated using a four-ssDNA crossover nanostructure that recruits split YFP when properly assembled. Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells." @default.
• W2338695783 created "2016-06-24" @default.
• W2338695783 creator A5014508768 @default.
• W2338695783 creator A5028170615 @default.
• W2338695783 creator A5040711703 @default.
• W2338695783 date "2016-04-19" @default.
• W2338695783 modified "2023-10-14" @default.
• W2338695783 title "Genetic encoding of DNA nanostructures and their self-assembly in living bacteria" @default.
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• W2338695783 doi "https://doi.org/10.1038/ncomms11179" @default.
• W2338695783 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4838831" @default.
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• W2338695783 hasPublicationYear "2016" @default.
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