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• W2338872238 abstract "Event Abstract Back to Event Electrical stimulation of cells in living bioelectronic devices Rachelle T. Hassarati1, 2, 3, L John R. Foster2, Maria L. Asplund3 and Rylie A. Green1 1 University of New South Wales, Graduate School of Biomedical Engineering, Australia 2 University of New South Wales, Bio/polymers Research Group, School of Biotechnology and Biomolecular Sciences, Australia 3 University of Freiburg, Department of Microsystems Engineering (IMTEK), Germany Introduction: Charge-balanced biphasic stimulation is commonly utilised in neuroprosthetic devices to initiate the required ionic changes that trigger neural cell depolarisation[1]. The alternating current direction associated with biphasic pulses reversal of chemical reactions which may have occurred at the electrode surface, however little is understood as to the influence the waveform will have on neural and glial cells seeded at the electrode interface of living bioelectronic devices. While some studies have shown that neural cells can extend processes along the gradient of electrical field potentials[2], few studies have sought to understand the impact on the supporting glia that provide growth factors and cell attachment sites for the neural cells. Olfactory ensheathing cells (OECs) are a glial cell type that has the potential to assist neural network formation in living electrode systems[3]. This study examined the impact of biphasic electrical stimulation (ES) on OEC survival and function, with respect to two electrode materials, conventional platinum (Pt) and low impedance, novel conductive hydrogel (CH). Materials and Methods: CHs were fabricated on Pt macroelectrodes as previously described in[3]. Briefly, poly(3,4-ethylenedioxythiophene) (PEDOT) was electropolymerised through a biosynthetic hydrogel consisting of poly(vinyl-alcohol) (PVA, 17 wt%) crosslinked with heparin (2 wt%), and gelatin (1 wt%). OECs were cultured on Pt and CH surfaces (1.2 x 104 cells/cm2) under proliferation conditions in full serum media for 48 h then subjected to one of two levels of biphasic ES (30 – 3000 µC/cm2, 1 h). 24 h post ES the OECs were harvested and image-based cytometry was used to determine the influence of ES on OEC viability and cell cycle. An indirect analysis of OEC functionality was also performed by co-culturing OECs with neural-like PC12 cells (in DMEM/F12 with 10% FBS, 1% PS). The ability of OECs to support neural cell differentiation when subjected to ES was determined via immunofluorescent analysis. Results and Discussion: OEC viability was high on both passive and stimulated samples. However, the lower voltages measured across the CH (147 ± 3 mV) compared to the Pt (317 ± 5 mV) showed the ability of these materials to transduce high stimulation currents with less impact on cell health, reflected by a higher percentage of viable cells on CH (91 - 93 %) compared to Pt (78 - 81 %). Cell cycle studies showed that the higher level of stimulation shifted the glial cells into a proliferative phase on both materials. Preliminary co-cultures of OECs and PC12s showed that the OECs under ES provided greater support of neural cell differentiation (Figure 1) suggesting that ES increases trophic factor and ECM support necessary for neural network development[4]. Figure 1. Quantification of neurite outgrowth density for PC12 cells cultured with and without OECs on passive and electrically stimulated Pt and CH electrodes. * shows significant differences for p < 0.05, Tukey one-way ANOVA. Error bars are standard error of the mean, n=3. Conclusion: Clinically relevant biphasic ES was shown to have a negligible impact on OEC viability, while shifting the cell cycle on either the Pt or CH coated electrodes into a more proliferative state. These cells have also demonstrated an ability to support neural cell differentiation under ES. Future work will seek to understand in greater detail the relationship between ES and glial cell output of neurotrophic factors. References:[1] Cogan, S.F., Neural stimulation and recording electrodes. Annu Rev Biomed Eng, 2008. 10: p. 275-309[2] Robinson, K.R., The responses of cells to electrical fields: a review. The Journal of cell biology, 1985. 101(6): p. 2023-2027.[3] Hassarati, R.T., et al., Biofunctionalization of conductive hydrogel coatings to support olfactory ensheathing cells at implantable electrode interfaces. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2015[4] Barros, C.S., S.J. Franco, and U. Müller, Extracellular matrix: functions in the nervous system. Cold Spring Harbor perspectives in biology, 2011. 3(1): p. a005108 Keywords: Hydrogel, stimuli-response, tissue compatibility, bioinerface Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Surface and interfacial characterization Citation: Hassarati RT, Foster LR, Asplund ML and Green RA (2016). Electrical stimulation of cells in living bioelectronic devices. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00455 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rachelle T Hassarati L John R Foster Maria L Asplund Rylie A Green Google Rachelle T Hassarati L John R Foster Maria L Asplund Rylie A Green Google Scholar Rachelle T Hassarati L John R Foster Maria L Asplund Rylie A Green PubMed Rachelle T Hassarati L John R Foster Maria L Asplund Rylie A Green Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2338872238 title "Electrical stimulation of cells in living bioelectronic devices" @default.
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