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- W233904634 abstract "Objective To detect the glycan profiling on the cell membrane of normal mucosal epithelial cells and gastric cancer cell lines GES-1,SGC7901,and MKN45 with lectin array and immunofluorescence assay.Methods The cells were digested with collagenase Ⅱ.The labeled cells were caught by lectins printed on the array according to distinctive glycan-binding specificities.The glycan profiling was scanned by laser fluorescence scanner.Results All the cells presented binding signals on most of lectin-binding sites,except for WBA,EEL and MAH-Ⅱ.For gastric cancer cell lines SGC7901 and MKN45,almost no binding signal was observed on the sites LTL and HHL.Conclusion According to the distinctive binding specificities of lectins,the common glycans on the cell membrane of these cell lines are sialic acid,N-acetylglucosamine/glucose,N-acetylgalactosamine/galactose,and mannose.In gastric cancer cell lines SGC7901 and MKN45,fucose is rarely expressed." @default.
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- W233904634 date "2009-01-01" @default.
- W233904634 modified "2023-09-25" @default.
- W233904634 title "Glycan profiling on the cell membrane of normal mucosal epithelial cells and gastric cancer cells detected with lectin array." @default.
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