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- W2339171856 abstract "ovalbumin(OVA)is a mRjor allergen of hnh egg WIllte and often induces hypersemitinty in atopIC children. Further kno叫 edge of the antigenic and allergenic determinants of OVA will allow better treatment to be provided for this disease. We investigated the antigenic determinants of OVA by immunoblotting and a microsequence analysis. OVA was digested 叫 th trypsin or chymotrypsin , and the p巴ptides were separated by tricine SDS-polyacrylamide gel electrophoresis. The antigenicity was tested by binding an anti-OVA rabbit IgG antibody with the peptides. Binding studies revealed that almost all bands of the tryptic peptides with MW of more than 5, 520 and almost all bands of the chymotryptic peptides with MW of mor巴 than 7, 280 were positiv巴. It is proposed that a part of the antigenic determinant existed in the OVA sequence of253-261 (or 264)" @default.
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- W2339171856 date "1998-03-15" @default.
- W2339171856 modified "2023-09-26" @default.
- W2339171856 title "Antigenic Peptides of Ovalbumin Digested with Trypsin or Chymotrypsin" @default.
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- W2339171856 doi "https://doi.org/10.11428/jhej1987.49.269" @default.
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