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- W2339222094 abstract "Sections of 6 μ from tissues fixed in Susa or in Bouin's fluid (without acetic acid) and embedded in paraffin were attached to slides with Mayer's albumen, dried at 37 C for 12 hr, deparaffinized and hydrated. The sections fixed in Susa were transferred to a I2-K1 solution (1:2:300 ml of water); rinsed in water, decolorized in 5% Na2S2O3; washed in running water, and rinsed in distilled water. Those fixed in Bouin's were transferred to 80% alcohol until decolorized, then rinsed in distilled water. All sections were stained in 1% aqueous phloxine, 10 min; rinsed in distilled water and transferred to 3% aqueous phosphotungstic acid, 1 min; rinsed in distilled water; stained 0.5 min in 0.05 azure II (Merck), washed in water; and finally, nuclear staining in Weigert's hematoxylin for 1 min was followed by a rinse in distilled water, rapid dehydration through alcohols, clearing in xylene and covering in balsam or a synthetic resin. In the completed stain, islet cells appear as follows: A cells, purple; B cells, weakly violet-blue; D cells, light blue with evident granules; exocrine cells, grayish blue with red granules." @default.
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- W2339222094 date "1967-01-01" @default.
- W2339222094 modified "2023-09-25" @default.
- W2339222094 title "A Phloxine-Azure-Hematoxylin Sequence for Differential Staining of Cells in Pancreatic Islets" @default.
- W2339222094 cites W4256465583 @default.
- W2339222094 doi "https://doi.org/10.3109/10520296709114975" @default.
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