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- W2339246146 abstract "In present study the developmental competence of sheep oocytes to reach maturation at second metaphase (M II) was observed following selection of oocytes using brilliant cressyl blue (BCB). Immature oocytes were harvested from ovaries collected at abattoir; the selected according to their colour appearence (cytoplasm colour) after being exposed to BCB and incubated for 90 minutes at 5% CO 2 incubator at 39 o C. The selected oocytes were grouped into two based on their cytoplsm colour i.e. group of oocytes (BCB+) with blue cytoplasm and growing oocytes (BCB-) the unstained cytoplasm. The control group including freshly collected oocytes which were then selected using routine method by observing morphological character under microscope. Each treated group of oocytes (BCB+ and BCB-) and the control were processed for maturation into culture media (Tissue Culture Medium199+10 IU/ml Pregnant Mare Serum Gonadothropine+10 IU Human Chorionic Gonadothropine+1µg/ml estradiol benzoat +10% fetal bovine serum) then incubated for 24 hours at 5% CO 2 incubator at 39 o C. Finally oocytes from each treated group and the control were stained with arceto orcein 2% to observe the number of oocytes which reach maturatuion at M II. The result showed that the percentage of oocytes reaching M II were significantly higher in BCB+ group (54%) compared to BCB- group (8%). It is concluded that BCB is a potential method for selectionof competent oocytes" @default.
- W2339246146 created "2016-06-24" @default.
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- W2339246146 date "2010-01-01" @default.
- W2339246146 modified "2023-09-27" @default.
- W2339246146 title "Seleksi Kemampuan Pematangan Oosit Domba Menggunakan Teknik Brilliant Cressyl Blue (SELECTING COMPETENCE SHEEP OOCYTES USING BRILLIANT CRESSYL BLUE)" @default.
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