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- W2339247705 abstract "Fructo-oligosaccharides are prebiotic and hypocaloric sweeteners that are usually extracted from chicory. They can also be produced from sucrose using fructosyltransferases, but the only commercial enzyme suitable for this purpose is Pectinex Ultra, which is produced with Aspergillus aculeatus. Here we used the yeast Kluyveromyces lactis to express a secreted recombinant fructosyltransferase from the inulin-producing fungus Aspergillus terreus. A synthetic codon-optimised version of the putative β-fructofuranosidase ATEG 04996 (XP 001214174.1) from A. terreus NIH2624 was secreted as a functional protein into the extracellular medium. At 60°C, the purified A. terreus enzyme generated the same pattern of oligosaccharides as Pectinex Ultra, but at lower temperatures it also produced oligomers with up to seven units. We achieved activities of up to 986.4 U/mL in high-level expression experiments, which is better than previous reports of optimised Aspergillus spp. fermentations." @default.
- W2339247705 created "2016-06-24" @default.
- W2339247705 creator A5039582983 @default.
- W2339247705 creator A5069118656 @default.
- W2339247705 date "2016-06-01" @default.
- W2339247705 modified "2023-10-17" @default.
- W2339247705 title "Heterologous expression of Aspergillus terreus fructosyltransferase in Kluyveromyces lactis" @default.
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- W2339247705 doi "https://doi.org/10.1016/j.nbt.2016.04.001" @default.
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