FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2339259944> ?p ?o ?g. }

• W2339259944 endingPage "598" @default.
• W2339259944 startingPage "591" @default.
• W2339259944 abstract "Annexins are a family of calcium-binding proteins that have been implicated in a wide range of intracellular processes. We have previously reported that stimulation of platelets with thrombin can induce the association of intracellular annexin V with membranes in two distinct ways. First, in such a way that it can be eluted from the membrane with EGTA and secondly in a manner such that it is tightly bound to the membrane and requires the non-ionic detergent Triton X-100 for its solubilization. We report that exposure of platelets to the calcium ionophore A23187 mimics the relocation induced by stimulation with thrombin. In separate experiments we demonstrate that a calcium ion concentration [Ca2+] of 0.8 microM is sufficient for maximum binding of the EGTA-resistant form to membranes. In contrast a higher [Ca2+] was required to induce maximal binding of the annexin V which could be extracted with EGTA. We demonstrate that following temperature-induced phase separation in Triton X-114, the membrane-associated annexin V partitions predominantly into the aqueous phase. We also show that the isoelectric point of annexin V does not change following membrane association. These observations suggest that a covalent modification, of annexin V itself, is not responsible for its association with the membrane. Millimolar [Ca2+] is required for maximal binding of purified annexin V to phospholipid vesicles. We show that binding to phospholipids can be reversed entirely by subsequent treatment with EGTA. This suggests that the EGTA-resistant form of annexin V is binding to a membrane component other than phosphatidylserine. Annexin V has previously been shown to bind to protein kinase C. Relocation of annexin V to membranes paralleled that of protein kinase C in thrombin-stimulated cells but not in cells treated with A23187, suggesting that these proteins are not functionally linked in platelet activation. Using bifunctional cross-linking reagents we have identified an 85 kDa complex containing annexin V. This may represent an association between annexin V and an annexin V-binding protein with a molecular mass of approximately 50 kDa." @default.
• W2339259944 created "2016-06-24" @default.
• W2339259944 creator A5019577580 @default.
• W2339259944 creator A5088011859 @default.
• W2339259944 creator A5089952263 @default.
• W2339259944 date "1995-06-01" @default.
• W2339259944 modified "2023-09-26" @default.
• W2339259944 title "Ca2+ concentration during binding determines the manner in which annexin V binds to membranes" @default.
• W2339259944 cites W101198430 @default.
• W2339259944 cites W1510923466 @default.
• W2339259944 cites W1544172591 @default.
• W2339259944 cites W1554938621 @default.
• W2339259944 cites W1582315378 @default.
• W2339259944 cites W1598566391 @default.
• W2339259944 cites W1605429420 @default.
• W2339259944 cites W1756031092 @default.
• W2339259944 cites W1883689801 @default.
• W2339259944 cites W1949772429 @default.
• W2339259944 cites W1959383367 @default.
• W2339259944 cites W1967058664 @default.
• W2339259944 cites W1969422658 @default.
• W2339259944 cites W1969926473 @default.
• W2339259944 cites W1977158442 @default.
• W2339259944 cites W1992310681 @default.
• W2339259944 cites W2004744930 @default.
• W2339259944 cites W2005194734 @default.
• W2339259944 cites W2007852891 @default.
• W2339259944 cites W2011442345 @default.
• W2339259944 cites W2015315729 @default.
• W2339259944 cites W2015874162 @default.
• W2339259944 cites W2018289835 @default.
• W2339259944 cites W2020977807 @default.
• W2339259944 cites W2023753439 @default.
• W2339259944 cites W2024929637 @default.
• W2339259944 cites W2041107578 @default.
• W2339259944 cites W2041181454 @default.
• W2339259944 cites W2049262981 @default.
• W2339259944 cites W2049558963 @default.
• W2339259944 cites W2051356917 @default.
• W2339259944 cites W2056291832 @default.
• W2339259944 cites W2073390867 @default.
• W2339259944 cites W2074321236 @default.
• W2339259944 cites W2079013578 @default.
• W2339259944 cites W2079295410 @default.
• W2339259944 cites W2079774792 @default.
• W2339259944 cites W2079930263 @default.
• W2339259944 cites W2081404575 @default.
• W2339259944 cites W2082375845 @default.
• W2339259944 cites W2085292543 @default.
• W2339259944 cites W2087996191 @default.
• W2339259944 cites W2091713185 @default.
• W2339259944 cites W2100837269 @default.
• W2339259944 cites W2101108802 @default.
• W2339259944 cites W2102686118 @default.
• W2339259944 cites W2113005146 @default.
• W2339259944 cites W2113225489 @default.
• W2339259944 cites W2120644189 @default.
• W2339259944 cites W2161651798 @default.
• W2339259944 cites W2163267116 @default.
• W2339259944 cites W2328607329 @default.
• W2339259944 cites W2409032288 @default.
• W2339259944 cites W2409114344 @default.
• W2339259944 cites W2418730364 @default.
• W2339259944 cites W24735528 @default.
• W2339259944 cites W309529471 @default.
• W2339259944 doi "https://doi.org/10.1042/bj3080591" @default.
• W2339259944 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1136967" @default.
• W2339259944 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7772046" @default.
• W2339259944 hasPublicationYear "1995" @default.
• W2339259944 type Work @default.
• W2339259944 sameAs 2339259944 @default.
• W2339259944 citedByCount "63" @default.
• W2339259944 countsByYear W23392599442012 @default.
• W2339259944 countsByYear W23392599442013 @default.
• W2339259944 countsByYear W23392599442015 @default.
• W2339259944 countsByYear W23392599442016 @default.
• W2339259944 countsByYear W23392599442017 @default.
• W2339259944 countsByYear W23392599442018 @default.
• W2339259944 countsByYear W23392599442020 @default.
• W2339259944 countsByYear W23392599442021 @default.
• W2339259944 countsByYear W23392599442022 @default.
• W2339259944 crossrefType "journal-article" @default.
• W2339259944 hasAuthorship W2339259944A5019577580 @default.
• W2339259944 hasAuthorship W2339259944A5088011859 @default.
• W2339259944 hasAuthorship W2339259944A5089952263 @default.
• W2339259944 hasBestOaLocation W23392599442 @default.
• W2339259944 hasConcept C12554922 @default.
• W2339259944 hasConcept C178790620 @default.
• W2339259944 hasConcept C181199279 @default.
• W2339259944 hasConcept C185592680 @default.
• W2339259944 hasConcept C202751555 @default.
• W2339259944 hasConcept C203014093 @default.
• W2339259944 hasConcept C2777292125 @default.
• W2339259944 hasConcept C2777658833 @default.
• W2339259944 hasConcept C2778918659 @default.
• W2339259944 hasConcept C2779014961 @default.
• W2339259944 hasConcept C2779637612 @default.
• W2339259944 hasConcept C30345302 @default.

• next