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- W2339334586 abstract "Previous studies have revealed the presence of multidrug resistant Enterobacteria in some processed convenience foods along the West African coast. The present study was carried out to determine the antibiotic sensitivity patterns and plasmid profiles of some of the Enterobacteria isolated from such foods in four West African countries. Antibiotic sensitivity testing was carried out to determine the sensitivity profiles of the isolates and the evidence for resistance plasmids was carried out by curing studies. Resistance plasmids were isolated using the alkaline phosphate method and the plasmid DNA was separated by electrophoresis on agarose gels. A total of forty three organisms were isolated and identified using standard physiological and biochemical methods. Generically the organisms were Citrobacter (6), Edwardsiella (2), Enterobacter (12), Escherichia coli (4), Klebsiella (4), Proteus (4), Salmonella (2), Serratia (2) and Shigella (7). Plasmid DNA was extracted from fourteen isolates i.e. four Shigella sp., two Salmonella sp., two Klebsiella sp., three Enterobacter sp., two Proteus sp. and one E. coli and it may be said that antibiotic resistance in these organisms were plasmid mediated. At the end of plasmid curing it was observed that sensitivity greatly increased. This study reveals that efforts being made by food standard regulatory bodies are inadequate to meet the Ready-to-eat food quality standards based on the sanitary levels. West Africa is a tourist destination hence high quality local market food is necessary and these findings would be useful in formulation of food-borne disease control and prevention strategies. Establishment and enforcement of a systematic and regular mechanism for surveillance and monitoring is needed to minimize the risks attached to consuming these foods." @default.
- W2339334586 created "2016-06-24" @default.
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- W2339334586 date "2016-01-01" @default.
- W2339334586 modified "2023-09-26" @default.
- W2339334586 title "Incidence and Plasmid Profiles of Multidrug Resistant Enterobacteria Isolates from Processed Convenience Foods in Some Countries of West Africa" @default.
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