FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2339843584> ?p ?o ?g. }

• W2339843584 endingPage "47" @default.
• W2339843584 startingPage "38" @default.
• W2339843584 abstract "In cultures of normal human epidermal keratinocytes (NHEKs), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces the expression of the epidermal growth factor receptor ligands transforming growth factor-α (TGF-α) and epiregulin (EREG). TCDD also down-regulates EGF receptors (EGFR), suggesting that decreases in signaling contribute to the effects of TCDD. In this study, we treated post-confluent NHEKs with 10 nM TCDD and assessed its effects on EGFR binding, EGFR ligand secretion, basal ERK activity, and proliferation. TCDD caused time-dependent deceases in [125I]-EGF binding to levels 78% of basal cell values at 72 h. Amphiregulin (AREG) levels increased with time in culture in basal and TCDD-treated cells, while TGF-α and epiregulin (EREG) secretion were stimulated by TCDD. Inhibiting EGFR ligand release with the metalloproteinase inhibitor batimastat prevented EGFR down-regulation and neutralizing antibodies for AREG and EREG relieved receptor down-regulation. In contrast, neutralizing TGF-α intensified EGFR down-regulation. Treating NHEKs with AREG or TGF-α caused rapid internalization of receptors with TGF-α promoting recycling within 90 min. EREG had limited effects on rapid internalization or recycling. TCDD treatment increased ERK activity, a response reduced by batimastat and the neutralization of all three ligands indicating that the EGFR and its ligands maintain ERK activity. All three EGFR ligands were required for the maintenance of total cell number in basal and TCDD-treated cultures. The EGFR inhibitor PD1530305 blocked basal and TCDD-induced increases in the number of cells labeled by 5-ethynyl-2'-deoxyuridine, identifying an EGFR-dependent pool of proliferating cells that is larger in TCDD-treated cultures. Overall, these data indicate that TCDD-induced EGFR down-regulation in NHEKs is caused by AREG, TGF-α, and EREG, while TGF-α enhances receptor recycling to maintain a pool of EGFR at the cell surface. These receptors are required for ERK activity, maintenance of total cell number, and stimulating the proliferation of a small subset cells." @default.
• W2339843584 created "2016-06-24" @default.
• W2339843584 creator A5013703078 @default.
• W2339843584 creator A5029095978 @default.
• W2339843584 creator A5034172336 @default.
• W2339843584 creator A5042997800 @default.
• W2339843584 creator A5046257042 @default.
• W2339843584 creator A5058407840 @default.
• W2339843584 date "2016-06-01" @default.
• W2339843584 modified "2023-09-26" @default.
• W2339843584 title "Role of EGF receptor ligands in TCDD-induced EGFR down-regulation and cellular proliferation" @default.
• W2339843584 cites W1453703257 @default.
• W2339843584 cites W1567276231 @default.
• W2339843584 cites W1579256405 @default.
• W2339843584 cites W1589488717 @default.
• W2339843584 cites W1607843768 @default.
• W2339843584 cites W1868042911 @default.
• W2339843584 cites W1963529390 @default.
• W2339843584 cites W1963544682 @default.
• W2339843584 cites W1966106548 @default.
• W2339843584 cites W1967957985 @default.
• W2339843584 cites W1969793246 @default.
• W2339843584 cites W1972814063 @default.
• W2339843584 cites W1984694084 @default.
• W2339843584 cites W1989946915 @default.
• W2339843584 cites W1992182416 @default.
• W2339843584 cites W1998224687 @default.
• W2339843584 cites W2000490940 @default.
• W2339843584 cites W2004698019 @default.
• W2339843584 cites W2004823364 @default.
• W2339843584 cites W2005630031 @default.
• W2339843584 cites W2018036562 @default.
• W2339843584 cites W2018612646 @default.
• W2339843584 cites W2018793834 @default.
• W2339843584 cites W2028496807 @default.
• W2339843584 cites W2054083814 @default.
• W2339843584 cites W2066378783 @default.
• W2339843584 cites W2068961038 @default.
• W2339843584 cites W2074313247 @default.
• W2339843584 cites W2078261279 @default.
• W2339843584 cites W2079556349 @default.
• W2339843584 cites W2089536120 @default.
• W2339843584 cites W2091017815 @default.
• W2339843584 cites W2091785370 @default.
• W2339843584 cites W2092619681 @default.
• W2339843584 cites W2093868559 @default.
• W2339843584 cites W2094540897 @default.
• W2339843584 cites W2104036338 @default.
• W2339843584 cites W2107495112 @default.
• W2339843584 cites W2107554012 @default.
• W2339843584 cites W2110771478 @default.
• W2339843584 cites W2119408080 @default.
• W2339843584 cites W2122643576 @default.
• W2339843584 cites W2134850272 @default.
• W2339843584 cites W2136194743 @default.
• W2339843584 cites W2139352112 @default.
• W2339843584 cites W2145545797 @default.
• W2339843584 cites W2149040307 @default.
• W2339843584 cites W2151339518 @default.
• W2339843584 cites W2153607649 @default.
• W2339843584 cites W2156775308 @default.
• W2339843584 cites W2158412843 @default.
• W2339843584 cites W2160926271 @default.
• W2339843584 cites W94162444 @default.
• W2339843584 doi "https://doi.org/10.1016/j.cbi.2016.04.031" @default.
• W2339843584 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4892999" @default.
• W2339843584 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27117977" @default.
• W2339843584 hasPublicationYear "2016" @default.
• W2339843584 type Work @default.
• W2339843584 sameAs 2339843584 @default.
• W2339843584 citedByCount "7" @default.
• W2339843584 countsByYear W23398435842018 @default.
• W2339843584 countsByYear W23398435842020 @default.
• W2339843584 countsByYear W23398435842023 @default.
• W2339843584 crossrefType "journal-article" @default.
• W2339843584 hasAuthorship W2339843584A5013703078 @default.
• W2339843584 hasAuthorship W2339843584A5029095978 @default.
• W2339843584 hasAuthorship W2339843584A5034172336 @default.
• W2339843584 hasAuthorship W2339843584A5042997800 @default.
• W2339843584 hasAuthorship W2339843584A5046257042 @default.
• W2339843584 hasAuthorship W2339843584A5058407840 @default.
• W2339843584 hasBestOaLocation W23398435842 @default.
• W2339843584 hasConcept C118131993 @default.
• W2339843584 hasConcept C125555471 @default.
• W2339843584 hasConcept C126322002 @default.
• W2339843584 hasConcept C134018914 @default.
• W2339843584 hasConcept C139770010 @default.
• W2339843584 hasConcept C159048773 @default.
• W2339843584 hasConcept C170493617 @default.
• W2339843584 hasConcept C185592680 @default.
• W2339843584 hasConcept C2775960820 @default.
• W2339843584 hasConcept C2776362946 @default.
• W2339843584 hasConcept C2777506169 @default.
• W2339843584 hasConcept C2777968868 @default.
• W2339843584 hasConcept C2779438470 @default.
• W2339843584 hasConcept C2780806702 @default.
• W2339843584 hasConcept C55493867 @default.
• W2339843584 hasConcept C57074206 @default.

• next