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• W2340169293 startingPage "1471" @default.
• W2340169293 abstract "Membrane transport P-type ATPases display two characteristic enzymatic activities: a principal ATPase activity provides the driving force for ion transport across biological membranes, whereas a promiscuous secondary activity catalyzes the hydrolysis of phosphate monoesters. This last activity is usually denoted as the phosphatase activity of P-ATPases. In the present study, we characterize the phosphatase activity of the Cu+-transport ATPase from Archaeglobus fulgidus (Af-CopA) and compare it with the principal ATPase activity. Our results show that the phosphatase turnover number was 20 times higher than that corresponding to the ATPase activity, but it is compensated by a high value of Km, producing a less efficient catalysis for pNPP. This secondary activity is enhanced by Mg2 + (essential activator) and phospholipids (non-essential activator), and inhibited by salts and Cu+. Transition state analysis of the catalyzed and noncatalyzed hydrolysis of pNPP indicates that Af-CopA enhances the reaction rates by a factor of 105 (ΔΔG‡ = 38 kJ/mol) mainly by reducing the enthalpy of activation (ΔΔH‡ = 30 kJ/mol), whereas the entropy of activation is less negative on the enzyme than in solution. For the ATPase activity, the decrease in the enthalpic component of the barrier is higher (ΔΔH‡ = 39 kJ/mol) and the entropic component is small on both the enzyme and in solution. These results suggest that different mechanisms are involved in the transference of the phosphoryl group of p-nitrophenyl phosphate and ATP." @default.
• W2340169293 created "2016-06-24" @default.
• W2340169293 creator A5019624088 @default.
• W2340169293 creator A5080918059 @default.
• W2340169293 date "2016-07-01" @default.
• W2340169293 modified "2023-10-14" @default.
• W2340169293 title "The promiscuous phosphomonoestearase activity of Archaeoglobus fulgidus CopA, a thermophilic Cu + transport ATPase" @default.
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• W2340169293 doi "https://doi.org/10.1016/j.bbamem.2016.04.006" @default.
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