FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2340211759> ?p ?o ?g. }

• W2340211759 abstract "Acoustophoresis revolutionized the field of container-less manipulation of liquids and solids by enabling mixing procedures which avoid contamination and loss of reagents due to the contact with the support. While its applications to chemistry and engineering are straightforward, additional developments are needed to obtain reliable biological protocols in a contactless environment. Here, we provide a first, fundamental step towards biological reactions in air by demonstrating the acoustophoretic DNA transfection of mammalian cells. We developed an original acoustophoretic design capable of levitating, moving and mixing biological suspensions of living mammalians cells and of DNA plasmids. The precise and sequential delivery of the mixed solutions into tissue culture plates is actuated by a novel mechanism based on the controlled actuation of the acoustophoretic force. The viability of the contactless procedure is tested using a cellular model sensitive to small perturbation of neuronal differentiation pathways. Additionally, the efficiency of the transfection procedure is compared to standard, container-based methods for both single and double DNA transfection and for different cell types including adherent growing HeLa cancer cells, and low adhesion neuron-like PC12 cells. In all, this work provides a proof of principle which paves the way to the development of high-throughput acoustophoretic biological reactors." @default.
• W2340211759 created "2016-06-24" @default.
• W2340211759 creator A5013514374 @default.
• W2340211759 creator A5029306551 @default.
• W2340211759 creator A5045460889 @default.
• W2340211759 creator A5074435194 @default.
• W2340211759 creator A5084204965 @default.
• W2340211759 date "2016-02-01" @default.
• W2340211759 modified "2023-10-18" @default.
• W2340211759 title "Toward Contactless Biology: Acoustophoretic DNA Transfection" @default.
• W2340211759 cites W1520821663 @default.
• W2340211759 cites W1543133300 @default.
• W2340211759 cites W1669562987 @default.
• W2340211759 cites W1964630494 @default.
• W2340211759 cites W1965861882 @default.
• W2340211759 cites W1973579525 @default.
• W2340211759 cites W1989974250 @default.
• W2340211759 cites W1997019056 @default.
• W2340211759 cites W1998645760 @default.
• W2340211759 cites W2013449127 @default.
• W2340211759 cites W2016876583 @default.
• W2340211759 cites W2021649658 @default.
• W2340211759 cites W2022397364 @default.
• W2340211759 cites W2022452275 @default.
• W2340211759 cites W2028715022 @default.
• W2340211759 cites W2033268594 @default.
• W2340211759 cites W2037483637 @default.
• W2340211759 cites W2038569615 @default.
• W2340211759 cites W2057263022 @default.
• W2340211759 cites W2064078501 @default.
• W2340211759 cites W2068388157 @default.
• W2340211759 cites W2071732315 @default.
• W2340211759 cites W2074966513 @default.
• W2340211759 cites W2077112895 @default.
• W2340211759 cites W2096041788 @default.
• W2340211759 cites W2102113498 @default.
• W2340211759 cites W2103535601 @default.
• W2340211759 cites W2107611782 @default.
• W2340211759 cites W2129046210 @default.
• W2340211759 cites W2142137317 @default.
• W2340211759 cites W2145896966 @default.
• W2340211759 cites W2148654471 @default.
• W2340211759 cites W2150895717 @default.
• W2340211759 cites W2169931542 @default.
• W2340211759 cites W2314562071 @default.
• W2340211759 cites W2399246465 @default.
• W2340211759 cites W2418672649 @default.
• W2340211759 cites W2554682736 @default.
• W2340211759 cites W4237768057 @default.
• W2340211759 cites W90997337 @default.
• W2340211759 doi "https://doi.org/10.1038/srep20023" @default.
• W2340211759 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4734324" @default.
• W2340211759 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26828312" @default.
• W2340211759 hasPublicationYear "2016" @default.
• W2340211759 type Work @default.
• W2340211759 sameAs 2340211759 @default.
• W2340211759 citedByCount "55" @default.
• W2340211759 countsByYear W23402117592017 @default.
• W2340211759 countsByYear W23402117592018 @default.
• W2340211759 countsByYear W23402117592019 @default.
• W2340211759 countsByYear W23402117592020 @default.
• W2340211759 countsByYear W23402117592021 @default.
• W2340211759 countsByYear W23402117592022 @default.
• W2340211759 countsByYear W23402117592023 @default.
• W2340211759 crossrefType "journal-article" @default.
• W2340211759 hasAuthorship W2340211759A5013514374 @default.
• W2340211759 hasAuthorship W2340211759A5029306551 @default.
• W2340211759 hasAuthorship W2340211759A5045460889 @default.
• W2340211759 hasAuthorship W2340211759A5074435194 @default.
• W2340211759 hasAuthorship W2340211759A5084204965 @default.
• W2340211759 hasBestOaLocation W23402117591 @default.
• W2340211759 hasConcept C147905033 @default.
• W2340211759 hasConcept C171250308 @default.
• W2340211759 hasConcept C185592680 @default.
• W2340211759 hasConcept C186060115 @default.
• W2340211759 hasConcept C192562407 @default.
• W2340211759 hasConcept C2984787389 @default.
• W2340211759 hasConcept C41008148 @default.
• W2340211759 hasConcept C54009773 @default.
• W2340211759 hasConcept C54355233 @default.
• W2340211759 hasConcept C552990157 @default.
• W2340211759 hasConcept C55493867 @default.
• W2340211759 hasConcept C81885089 @default.
• W2340211759 hasConcept C8673954 @default.
• W2340211759 hasConcept C86803240 @default.
• W2340211759 hasConcept C95444343 @default.
• W2340211759 hasConceptScore W2340211759C147905033 @default.
• W2340211759 hasConceptScore W2340211759C171250308 @default.
• W2340211759 hasConceptScore W2340211759C185592680 @default.
• W2340211759 hasConceptScore W2340211759C186060115 @default.
• W2340211759 hasConceptScore W2340211759C192562407 @default.
• W2340211759 hasConceptScore W2340211759C2984787389 @default.
• W2340211759 hasConceptScore W2340211759C41008148 @default.
• W2340211759 hasConceptScore W2340211759C54009773 @default.
• W2340211759 hasConceptScore W2340211759C54355233 @default.
• W2340211759 hasConceptScore W2340211759C552990157 @default.
• W2340211759 hasConceptScore W2340211759C55493867 @default.
• W2340211759 hasConceptScore W2340211759C81885089 @default.
• W2340211759 hasConceptScore W2340211759C8673954 @default.
• W2340211759 hasConceptScore W2340211759C86803240 @default.

• next