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• W2340261034 endingPage "427" @default.
• W2340261034 startingPage "421" @default.
• W2340261034 abstract "Contents Lead (Pb 2+ ) is a toxic heavy metal which interferes with several physiological processes regulated by Ca 2+ , including those characterized by changes of the membrane stability and the motility of spermatozoa necessary for the fertilization of the oocyte. In this study, ejaculated sperm from six rams ( Ovis aries ) have been incubated in vitro with or without 50 ng Pb 2+ /ml during 30 min and in the presence or absence of three different potential modulators of the effects of Pb 2+ on changes in the sperm membrane before fertilization: charybdotoxin, quinacrine and staurosporine. Sperm samples incubated with Pb 2+ have shown significant reductions in acrosome integrity and sperm viability and an increase in progressive movement. None of the studied potential modulators had a protective effect against Pb 2+ action. On the contrary, Pb 2+ ‐incubated sperm in the presence of staurosporine had lower acrosome integrity, and lower sperm viability was observed when spermatozoa were incubated with Pb 2+ + charybdotoxin. Quinacrine was the only tested substance capable of increasing the concentration of Pb 2+ in spermatozoa; thus, the enhancement of Pb 2+ effects produced by staurosporine and charybdotoxin was not produced by an increased uptake of Pb 2+ by spermatozoa. However, the increase of intracellular Pb 2+ in those spermatozoa incubated with quinacrine did not result in an adverse effect on sperm motility or viability although the acrosome integrity was negatively affected." @default.
• W2340261034 created "2016-06-24" @default.
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• W2340261034 date "2016-04-20" @default.
• W2340261034 modified "2023-09-26" @default.
• W2340261034 title "An<i>In Vitro</i>Evaluation of Biochemical Processes Involved in Lead-Induced Changes on Ram Spermatozoa" @default.
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• W2340261034 doi "https://doi.org/10.1111/rda.12696" @default.
• W2340261034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27095430" @default.
• W2340261034 hasPublicationYear "2016" @default.
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