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- W2340559944 abstract "Nox1 is an abundant source of reactive oxygen species (ROS) in colon epithelium recently shown to function in wound healing and epithelial homeostasis. We identified Peroxiredoxin 6 (Prdx6) as a novel binding partner of Nox activator 1 (Noxa1) in yeast two-hybrid screening experiments using the Noxa1 SH3 domain as bait. Prdx6 is a unique member of the Prdx antioxidant enzyme family exhibiting both glutathione peroxidase and phospholipase A2 activities. We confirmed this interaction in cells overexpressing both proteins, showing Prdx6 binds to and stabilizes wild type Noxa1, but not the SH3 domain mutant form, Noxa1 W436R. We demonstrated in several cell models that Prdx6 knockdown suppresses Nox1 activity, whereas enhanced Prdx6 expression supports higher Nox1-derived superoxide production. Both peroxidase- and lipase-deficient mutant forms of Prdx6 (Prdx6 C47S and S32A, respectively) failed to bind to or stabilize Nox1 components or support Nox1-mediated superoxide generation. Furthermore, the transition-state substrate analogue inhibitor of Prdx6 phospholipase A2 activity (MJ-33) was shown to suppress Nox1 activity, suggesting Nox1 activity is regulated by the phospholipase activity of Prdx6. Finally, wild type Prdx6, but not lipase or peroxidase mutant forms, supports Nox1-mediated cell migration in the HCT-116 colon epithelial cell model of wound closure. These findings highlight a novel pathway in which this antioxidant enzyme positively regulates an oxidant-generating system to support cell migration and wound healing." @default.
- W2340559944 created "2016-06-24" @default.
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- W2340559944 date "2016-07-01" @default.
- W2340559944 modified "2023-09-30" @default.
- W2340559944 title "Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration" @default.
- W2340559944 cites W1892259311 @default.
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- W2340559944 cites W1976655661 @default.
- W2340559944 cites W1977186059 @default.
- W2340559944 cites W1977298095 @default.
- W2340559944 cites W1985033865 @default.
- W2340559944 cites W1991825280 @default.
- W2340559944 cites W1997619660 @default.
- W2340559944 cites W2003241255 @default.
- W2340559944 cites W2009707750 @default.
- W2340559944 cites W2009895452 @default.
- W2340559944 cites W2012181651 @default.
- W2340559944 cites W2013280333 @default.
- W2340559944 cites W2014152577 @default.
- W2340559944 cites W2016241657 @default.
- W2340559944 cites W2016839775 @default.
- W2340559944 cites W2019437029 @default.
- W2340559944 cites W2020319663 @default.
- W2340559944 cites W2022385815 @default.
- W2340559944 cites W2023064969 @default.
- W2340559944 cites W2027105670 @default.
- W2340559944 cites W2027983298 @default.
- W2340559944 cites W2035040393 @default.
- W2340559944 cites W2035666481 @default.
- W2340559944 cites W2036811549 @default.
- W2340559944 cites W2040280663 @default.
- W2340559944 cites W2041317319 @default.
- W2340559944 cites W2045549203 @default.
- W2340559944 cites W2055939934 @default.
- W2340559944 cites W2057635215 @default.
- W2340559944 cites W2057893902 @default.
- W2340559944 cites W2060089822 @default.
- W2340559944 cites W2060273459 @default.
- W2340559944 cites W2060762539 @default.
- W2340559944 cites W2061234580 @default.
- W2340559944 cites W2067374348 @default.
- W2340559944 cites W2067413723 @default.
- W2340559944 cites W2067773703 @default.
- W2340559944 cites W2073364986 @default.
- W2340559944 cites W2075778216 @default.
- W2340559944 cites W2089818796 @default.
- W2340559944 cites W2101745219 @default.
- W2340559944 cites W2104541176 @default.
- W2340559944 cites W2108123444 @default.
- W2340559944 cites W2109584029 @default.
- W2340559944 cites W2109997609 @default.
- W2340559944 cites W2110606487 @default.
- W2340559944 cites W2113202066 @default.
- W2340559944 cites W2121474571 @default.
- W2340559944 cites W2135028625 @default.
- W2340559944 cites W2135445808 @default.
- W2340559944 cites W2135841986 @default.
- W2340559944 cites W2143659065 @default.
- W2340559944 cites W2146314181 @default.
- W2340559944 cites W2150005311 @default.
- W2340559944 cites W2150591923 @default.
- W2340559944 cites W2152736066 @default.
- W2340559944 cites W2153437220 @default.
- W2340559944 cites W2158429932 @default.
- W2340559944 cites W2160589469 @default.
- W2340559944 cites W2167040135 @default.
- W2340559944 cites W2169549805 @default.
- W2340559944 cites W2320703070 @default.
- W2340559944 cites W2335847907 @default.
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- W2340559944 doi "https://doi.org/10.1016/j.freeradbiomed.2016.04.009" @default.
- W2340559944 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4929831" @default.
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- W2340559944 hasPublicationYear "2016" @default.
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