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- W2340633014 abstract "Objective To construct the DNA vaccine against highly pathogenic human-avian influenza H5N1 virus and observe its immunogenicity.Methods The phylogenetic relationship of HA gene of human-avian influenza H5N1 virus was analyzed,based on which the HA gene containing the common sequences of most of human-avian influenza H5N1 virus strains was synthesized,and recombinant plasmids pVAX-CLHA carrying cytotoxic T lymphocyte antigen 4(CTLA4)-HA fusion gene and pVAX-HA carrying HA gene were constructed then,after identification by restriction analysis and sequencing,transfected to 293-T cells.The transcription of HA mRNA in transfected cells was determined by RT-PCR,and the expression of HA by IFA.BALB/c mice were immunized with recombinant plasmids pVAX-CLHA and pVAX-HA respectively,and the HI antibody titers in sera were determined.Results Restriction analysis and sequencing proved that recombinant plasmids pVAX-CLHA and pVAX-HA were constructed correctly.The transcription of target gene and expression of target protein were observed in the 293-T cells transfected.The immunization with either recombinant plasmid pVAX-CLHA or pVAX-HA for 3 times induced HI antibodies in BALB/c mice,however,the HI antibody titer induced by pVAX-CLHA was higher than that by pVAX-HA.Conclusion The DNA vaccine plasmid carrying the fusion gene of H5N1 HA and CTLA4 was successfully constructed and showed good immune effect in mice,which laid a foundation of development of DNA vaccine against highly pathogenic human-avian influenza." @default.
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- W2340633014 date "2010-01-01" @default.
- W2340633014 modified "2023-09-23" @default.
- W2340633014 title "Construction and Immunogenicity of Highly Pathogenic Human-Avian Influenza H5N1 Virus DNA Vaccine" @default.
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