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• W2340725211 abstract "A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs). Au NPs could not only attach abundant luminol for the enhancement of ECL signal, but also provide a friendly microenvironment for the immobilization of antibodies. Moreover, 1-butylpyridinium tetrafluroborate ([BPy]BF4) were used to disperse luminol functional-Au NPs@polypyrrole nanocomposites, resulting in the film-formation of composites on the electrode, which could improve the stability of immunosensor. In particular, employment of single-step cycle pulse could limit the consecutive reaction between luminol and H2O2 efficiently, thus leading to stable and strong signals. The proposed method presents good ECL response for the detection of CEA allowing a wide linear range from 0.01 pg/mL to 10 ng/mL and a limit of detection as low as 3 fg/mL. The immunosensor would be a promising tool in the early diagnosis of CEA due to its high sensitivity, simplicity and cost-effective." @default.
• W2340725211 created "2016-06-24" @default.
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• W2340725211 date "2016-04-19" @default.
• W2340725211 modified "2023-09-24" @default.
• W2340725211 title "Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection" @default.
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• W2340725211 doi "https://doi.org/10.1038/srep24599" @default.
• W2340725211 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4835776" @default.
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