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• W2340735502 abstract "Sponges host complex microbial communities of recognized ecological and biotechnological importance. Extensive cultivation efforts have been made to isolate sponge bacteria, but most still elude cultivation. To identify the bottlenecks of sponge bacterial cultivation, we combined high-throughput 16S rRNA gene sequencing with a variety of cultivation media and incubation conditions. We aimed to determine the extent to which sample processing and cultivation conditions can impact bacterial viability and recovery in culture. We isolated 325 sponge bacteria from 6 specimens of Cymbastela concentrica and 3 specimens of Scopalina sp.. These isolates were distributed over 37 different genera and 47 operational taxonomic units (defined at 97% 16S rRNA gene sequence identity). The cultivable bacterial community was highly specific to its sponge host and different media compositions yielded distinct microbial isolates. Around 97% of the isolates could be detected in the original sponge and represented a large but highly variable proportion (0.5% to 92% total abundance, depending on sponge species) of viable bacteria obtained after sample processing, as determined by propidium monoazide (PMA) selective DNA modification of compromised cells. Our results show that the most abundant viable bacteria are also the most predominant groups found in cultivation, reflecting, to some extent, the relative abundances of the viable bacterial community, rather than the overall community estimated by direct molecular approaches. Cultivation is therefore shaped not only by the growth conditions provided, but also by the different cell viabilities of the bacteria that constitute the cultivation inoculum. These observations highlight the need to perform experiments to assess each method of sample processing for its accurate representation of the actual in situ bacterial community and its yield of viable cells." @default.
• W2340735502 created "2016-06-24" @default.
• W2340735502 creator A5042700117 @default.
• W2340735502 creator A5043304229 @default.
• W2340735502 creator A5058094721 @default.
• W2340735502 creator A5088030110 @default.
• W2340735502 date "2016-04-12" @default.
• W2340735502 modified "2023-10-05" @default.
• W2340735502 title "Sample Processing Impacts the Viability and Cultivability of the Sponge Microbiome" @default.
• W2340735502 cites W1503066550 @default.
• W2340735502 cites W1548461904 @default.
• W2340735502 cites W1553056762 @default.
• W2340735502 cites W1564664469 @default.
• W2340735502 cites W1578897783 @default.
• W2340735502 cites W1583109083 @default.
• W2340735502 cites W1594388293 @default.
• W2340735502 cites W1606930879 @default.
• W2340735502 cites W1625659755 @default.
• W2340735502 cites W1634598038 @default.
• W2340735502 cites W181370739 @default.
• W2340735502 cites W1849874345 @default.
• W2340735502 cites W1854367204 @default.
• W2340735502 cites W1895548850 @default.
• W2340735502 cites W1966018744 @default.
• W2340735502 cites W1970958609 @default.
• W2340735502 cites W1974935006 @default.
• W2340735502 cites W1975778959 @default.
• W2340735502 cites W1977547664 @default.
• W2340735502 cites W1984094408 @default.
• W2340735502 cites W1984473712 @default.
• W2340735502 cites W1985898172 @default.
• W2340735502 cites W1988925586 @default.
• W2340735502 cites W1990953278 @default.
• W2340735502 cites W1991388144 @default.
• W2340735502 cites W1996758006 @default.
• W2340735502 cites W1998024303 @default.
• W2340735502 cites W2000628097 @default.
• W2340735502 cites W2007404630 @default.
• W2340735502 cites W2008153803 @default.
• W2340735502 cites W2012617060 @default.
• W2340735502 cites W2018544633 @default.
• W2340735502 cites W2020222675 @default.
• W2340735502 cites W2023764846 @default.
• W2340735502 cites W2025210407 @default.
• W2340735502 cites W2034285706 @default.
• W2340735502 cites W2040424745 @default.
• W2340735502 cites W2042631505 @default.
• W2340735502 cites W2042715699 @default.
• W2340735502 cites W2048824740 @default.
• W2340735502 cites W2049632519 @default.
• W2340735502 cites W2053297344 @default.
• W2340735502 cites W2053655213 @default.
• W2340735502 cites W2056836855 @default.
• W2340735502 cites W2061046203 @default.
• W2340735502 cites W2063155474 @default.
• W2340735502 cites W2063187023 @default.
• W2340735502 cites W2063764670 @default.
• W2340735502 cites W2068467616 @default.
• W2340735502 cites W2076227545 @default.
• W2340735502 cites W2079094106 @default.
• W2340735502 cites W2079745377 @default.
• W2340735502 cites W2082790034 @default.
• W2340735502 cites W2085520882 @default.
• W2340735502 cites W2091113559 @default.
• W2340735502 cites W2098393431 @default.
• W2340735502 cites W2100359842 @default.
• W2340735502 cites W2102424000 @default.
• W2340735502 cites W2103456457 @default.
• W2340735502 cites W2106069449 @default.
• W2340735502 cites W2108209129 @default.
• W2340735502 cites W2108718991 @default.
• W2340735502 cites W2111955985 @default.
• W2340735502 cites W2119010870 @default.
• W2340735502 cites W2121046581 @default.
• W2340735502 cites W2122545928 @default.
• W2340735502 cites W2122985424 @default.
• W2340735502 cites W2129553172 @default.
• W2340735502 cites W2129610424 @default.
• W2340735502 cites W2136879569 @default.
• W2340735502 cites W2139876538 @default.
• W2340735502 cites W2140682071 @default.
• W2340735502 cites W2147378425 @default.
• W2340735502 cites W2148165415 @default.
• W2340735502 cites W2148970424 @default.
• W2340735502 cites W2151350595 @default.
• W2340735502 cites W2152093511 @default.
• W2340735502 cites W2152885278 @default.
• W2340735502 cites W2154401636 @default.
• W2340735502 cites W2157929705 @default.
• W2340735502 cites W2164110299 @default.
• W2340735502 cites W2166171121 @default.
• W2340735502 cites W2169637929 @default.
• W2340735502 cites W2170968801 @default.
• W2340735502 cites W2176371198 @default.
• W2340735502 doi "https://doi.org/10.3389/fmicb.2016.00499" @default.
• W2340735502 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4876369" @default.
• W2340735502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27242673" @default.
• W2340735502 hasPublicationYear "2016" @default.
• W2340735502 type Work @default.
• W2340735502 sameAs 2340735502 @default.

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