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• W2340821372 startingPage "66" @default.
• W2340821372 abstract "Functionalized giant unilamellar vesicles (GUVs) containing a fluorescence dye Rhodamine 6G is proposed as a marker in sandwich-type immunoassay for bovine serum albumin (BSA) and lipocalin-2 (LCN2). The GUVs were prepared by the electroformation method and functionalized with anti-BSA antibody and anti-LCN2 antibody, respectively. The purification of antibody-modified GUVs was achieved by conventional centrifugation and a washing step in a flow system. To antigen on an antibody slip, antibody-modified GUVs were added as a marker and incubated. After wash-out of excess reagents and lysis of the bound GUVs with Triton X-100, the fluorescence image was captured. The fluorometric immunoassays for BSA and LCN2 exhibited lower detection limits of 4 and 80 fg ml−1, respectively." @default.
• W2340821372 created "2016-06-24" @default.
• W2340821372 creator A5007354621 @default.
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• W2340821372 date "2016-07-01" @default.
• W2340821372 modified "2023-09-27" @default.
• W2340821372 title "Giant unilamellar vesicles containing Rhodamine 6G as a marker for immunoassay of bovine serum albumin and lipocalin-2" @default.
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• W2340821372 doi "https://doi.org/10.1016/j.ab.2016.04.011" @default.
• W2340821372 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27117116" @default.
• W2340821372 hasPublicationYear "2016" @default.
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