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- W2341250780 abstract "There are many sequencing-based approaches to understanding complex metagenomic communities spanning targeted amplification to whole-sample shotgun sequencing. While targeted approaches provide valuable data at low sequencing depth, they are limited by primer design and PCR amplification. Wholesample shotgun experiments generally use short-read, second-generation sequencing, which results in data processing difficulties. For example, reads less than 1 Kb in length will likely not cover a complete gene or region of interest, and will require assembly. This not only introduces the possibility of incorrectly combining sequence from different community members, it requires a high depth of coverage. As such, rare community members may not be represented in the resulting assembly. Circular-consensus, single molecule, real-time (SMRT®) Sequencing reads in the 1-2 kb range, with >99% accuracy can be efficiently generated for low amounts of input DNA. 10 ng of input DNA sequenced in 4 SMRT Cells would generate >100,000 such reads. While throughput is low compared to second-generation sequencing, the reads are a true random sampling of the underlying community, since SMRT Sequencing has been shown to have no sequence-context bias. Long read lengths mean that that it would be reasonable to expect a high number of the reads to include gene fragments useful for analysis. Introduction BEI Mock Staggered Community Clostridium difficile Patient, Fecal Microbiota Transplant (FMT) Richard J. Hall1, Cheryl A. Gale2, Matt Hamilton3, Tim Heiseland3, Alex Knotted3, Michael Sadowsky3, Kevin Silverstein4, Alexa Weingarden3 and Cheryl Heiner1 1Pacific Biosciences, Menlo Park, CA, 2Department of Pediatrics, University of Minnesota, 3BioTechnology Institute, University of Minnesota, 4Supercomputing Institute for Advanced Computational Research, University of Minnesota" @default.
- W2341250780 created "2016-06-24" @default.
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- W2341250780 date "2015-01-01" @default.
- W2341250780 modified "2023-09-27" @default.
- W2341250780 title "Profiling Metagenomic Communities Using Circular Consensus and Single Molecule, Real-Time Sequencing" @default.
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