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• W2341264215 endingPage "e0153218" @default.
• W2341264215 startingPage "e0153218" @default.
• W2341264215 abstract "Clubroot, caused by Plasmodiophora brassicae, is an important disease on Brassica species worldwide. A clubroot resistance gene, Rcr1, with efficacy against pathotype 3 of P. brassicae, was previously mapped to chromosome A03 of B. rapa in pak choy cultivar “Flower Nabana”. In the current study, resistance to pathotypes 2, 5 and 6 was shown to be associated with Rcr1 region on chromosome A03. Bulked segregant RNA sequencing was performed and short read sequences were assembled into 10 chromosomes of the B. rapa reference genome v1.5. For the resistant (R) bulks, a total of 351.8 million (M) sequences, 30,836.5 million bases (Mb) in length, produced 120-fold coverage of the reference genome. For the susceptible (S) bulks, 322.9 M sequences, 28,216.6 Mb in length, produced 109-fold coverage. In total, 776.2 K single nucleotide polymorphisms (SNPs) and 122.2 K insertion / deletion (InDels) in R bulks and 762.8 K SNPs and 118.7 K InDels in S bulks were identified; each chromosome had about 87% SNPs and 13% InDels, with 78% monomorphic and 22% polymorphic variants between the R and S bulks. Polymorphic variants on each chromosome were usually below 23%, but made up 34% of the variants on chromosome A03. There were 35 genes annotated in the Rcr1 target region and variants were identified in 21 genes. The numbers of poly variants differed significantly among the genes. Four out of them encode Toll-Interleukin-1 receptor / nucleotide-binding site / leucine-rich-repeat proteins; Bra019409 and Bra019410 harbored the higher numbers of polymorphic variants, which indicates that they are more likely candidates of Rcr1. Fourteen SNP markers in the target region were genotyped using the Kompetitive Allele Specific PCR method and were confirmed to associate with Rcr1. Selected SNP markers were analyzed with 26 recombinants obtained from a segregating population consisting of 1587 plants, indicating that they were completely linked to Rcr1. Nine SNP markers were used for marker-assisted introgression of Rcr1 into B. napus canola from B. rapa, with 100% accuracy in this study." @default.
• W2341264215 created "2016-06-24" @default.
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• W2341264215 date "2016-04-14" @default.
• W2341264215 modified "2023-10-16" @default.
• W2341264215 title "Identification of Genome-Wide Variants and Discovery of Variants Associated with Brassica rapa Clubroot Resistance Gene Rcr1 through Bulked Segregant RNA Sequencing" @default.
• W2341264215 cites W1564594770 @default.
• W2341264215 cites W1582224782 @default.
• W2341264215 cites W1953254900 @default.
• W2341264215 cites W1964903839 @default.
• W2341264215 cites W1966286620 @default.
• W2341264215 cites W1968713994 @default.
• W2341264215 cites W1969584121 @default.
• W2341264215 cites W1974460471 @default.
• W2341264215 cites W1974511707 @default.
• W2341264215 cites W1975206298 @default.
• W2341264215 cites W1976919046 @default.
• W2341264215 cites W1978615046 @default.
• W2341264215 cites W1985312077 @default.
• W2341264215 cites W1986446099 @default.
• W2341264215 cites W1987105664 @default.
• W2341264215 cites W1987806159 @default.
• W2341264215 cites W1992199290 @default.
• W2341264215 cites W1993096431 @default.
• W2341264215 cites W1997613662 @default.
• W2341264215 cites W1999102617 @default.
• W2341264215 cites W2023031078 @default.
• W2341264215 cites W2023320089 @default.
• W2341264215 cites W2025557847 @default.
• W2341264215 cites W2028027287 @default.
• W2341264215 cites W2030647412 @default.
• W2341264215 cites W2032698912 @default.
• W2341264215 cites W2033810712 @default.
• W2341264215 cites W2033973888 @default.
• W2341264215 cites W2036138299 @default.
• W2341264215 cites W2037109441 @default.
• W2341264215 cites W2039641796 @default.
• W2341264215 cites W2044986424 @default.
• W2341264215 cites W2045966516 @default.
• W2341264215 cites W2057316183 @default.
• W2341264215 cites W2057647603 @default.
• W2341264215 cites W2060374222 @default.
• W2341264215 cites W2060609869 @default.
• W2341264215 cites W2063905531 @default.
• W2341264215 cites W2064025795 @default.
• W2341264215 cites W2067286468 @default.
• W2341264215 cites W2069793050 @default.
• W2341264215 cites W2070714761 @default.
• W2341264215 cites W2072715465 @default.
• W2341264215 cites W2077620253 @default.
• W2341264215 cites W2078278976 @default.
• W2341264215 cites W2079371733 @default.
• W2341264215 cites W2083049920 @default.
• W2341264215 cites W2083759168 @default.
• W2341264215 cites W2083761531 @default.
• W2341264215 cites W2084123518 @default.
• W2341264215 cites W2086338036 @default.
• W2341264215 cites W2086829335 @default.
• W2341264215 cites W2086895026 @default.
• W2341264215 cites W2089085193 @default.
• W2341264215 cites W2089505986 @default.
• W2341264215 cites W2090759994 @default.
• W2341264215 cites W2095133079 @default.
• W2341264215 cites W2105938072 @default.
• W2341264215 cites W2106260006 @default.
• W2341264215 cites W2113280067 @default.
• W2341264215 cites W2141550398 @default.
• W2341264215 cites W2145203699 @default.
• W2341264215 cites W2145396130 @default.
• W2341264215 cites W2149340380 @default.
• W2341264215 cites W2151649236 @default.
• W2341264215 cites W2159615540 @default.
• W2341264215 cites W2160445799 @default.
• W2341264215 cites W2332757557 @default.
• W2341264215 cites W2470324699 @default.
• W2341264215 cites W2471022341 @default.
• W2341264215 cites W2590912527 @default.
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• W2341264215 doi "https://doi.org/10.1371/journal.pone.0153218" @default.
• W2341264215 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4831815" @default.
• W2341264215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27078023" @default.
• W2341264215 hasPublicationYear "2016" @default.
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