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- W2341411406 abstract "To construct a recombinant bovine herpesvirus 1 (BHV-1) expressing the G protein gene of bovine respiratory syncytial virus (BRSV), a BHV-1 transfer vector with flanking BHV-1 TK gene was constructed by inserting a synthetic G protein gene sequence of BRSV under the control of CMV promoter. The transfer vector and the parental recombinant virus (BHV-1/TK-/Lac Z+) DNA were co-transfected into BT cells using calcium phosphate-mediated transfection. The recombinant BHV-1 (designated as BHV-1/TK-/G+) was obtained by selection for white virus plaques in presence of X-gal. PCR results showed that G gene was successfully inserted into the genome of BHV-1/TK-/Lac Z+ and replaced the LacZ gene. The expression of G protein in infected cells was confirmed by indirect immunofluorescence assay and western blot. This work provided a basis for development of BHV-1 vector vaccines for BRSV and other important bovine infectious diseases." @default.
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- W2341411406 date "2010-01-01" @default.
- W2341411406 modified "2023-09-22" @default.
- W2341411406 title "Construction and identification of a recombinant BHV-1 expressing the G protein gene of bovine respiratory syncytial virus." @default.
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