FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2342703009> ?p ?o ?g. }

• W2342703009 endingPage "F1069" @default.
• W2342703009 startingPage "F1060" @default.
• W2342703009 abstract "In a lentivirus-based gene delivery system, the incorporated gene is continuously expressed for a long time. In this study, we devised a simple way to knock down a specific gene in a kidney cell-specific pattern in adult mice by lentivirus-assisted transfer of short hairpin RNA (shRNA). Kidney collecting duct (CD)-specific aquaporin-3 (AQP3)-knockdown mice were generated by consecutive injection of Hoxb7-Cre-expressing lentivirus (LV-Hoxb7 Cre) and loxP-AQP3 shRNA-expressing lentivirus (LV-loxP shAQP3) in adult C57BL6/J mice. LV-Hoxb7 Cre was designed to express mCherry, while LV-loxP shAQP3 was designed with a floxed enhanced green fluorescent protein (EGFP)-tagged stop sequence, and thus EGFP would be expressed only in the absence of Cre recombination. In mice treated with LV-Hoxb7 Cre alone, mCherry protein expression, which indicates the presence of Cre recombinase, occurred only in CD cells. However, LV-loxP shAQP3 injection alone resulted in an increase in EGFP expression in all kidney cells, indicating the transcription of the floxed region. When LV-Hoxb7 Cre and LV-loxP shAQP3 were sequentially transduced, EGFP expression was attenuated while mCherry expression was sustained in CD cells, demonstrating a CD cell-specific recombination of the floxed region. AQP3 expression in mice injected with LV-Hoxb7 Cre or LV-loxP shAQP3 alone did not differ, but consecutive injection of LV-Hoxb7 Cre and LV-loxP shAQP3 significantly reduced AQP3 expression in CD cells. However, the expression levels of AQP3 were not altered in other cell types. Double transduction of Cre- and loxP-based lentivirus can easily generate kidney cell-specific knockdown mice, and this method might be applicable to other species." @default.
• W2342703009 created "2016-06-24" @default.
• W2342703009 creator A5013151848 @default.
• W2342703009 creator A5021592381 @default.
• W2342703009 creator A5033449275 @default.
• W2342703009 creator A5034116139 @default.
• W2342703009 creator A5044459999 @default.
• W2342703009 creator A5047082140 @default.
• W2342703009 creator A5050687492 @default.
• W2342703009 creator A5057443708 @default.
• W2342703009 creator A5084723267 @default.
• W2342703009 creator A5085406568 @default.
• W2342703009 creator A5085491969 @default.
• W2342703009 creator A5090275275 @default.
• W2342703009 date "2015-12-15" @default.
• W2342703009 modified "2023-10-18" @default.
• W2342703009 title "Double transduction of a Cre/LoxP lentiviral vector: a simple method to generate kidney cell-specific knockdown mice" @default.
• W2342703009 cites W1505088608 @default.
• W2342703009 cites W1963756597 @default.
• W2342703009 cites W1967287827 @default.
• W2342703009 cites W1971643509 @default.
• W2342703009 cites W1972160020 @default.
• W2342703009 cites W1990710362 @default.
• W2342703009 cites W1990906839 @default.
• W2342703009 cites W1992251016 @default.
• W2342703009 cites W1994075097 @default.
• W2342703009 cites W1994921822 @default.
• W2342703009 cites W2000774201 @default.
• W2342703009 cites W2002316686 @default.
• W2342703009 cites W2005275397 @default.
• W2342703009 cites W2010243546 @default.
• W2342703009 cites W2014297716 @default.
• W2342703009 cites W2019893496 @default.
• W2342703009 cites W2020024953 @default.
• W2342703009 cites W2034450106 @default.
• W2342703009 cites W2034640715 @default.
• W2342703009 cites W2038970416 @default.
• W2342703009 cites W2048139403 @default.
• W2342703009 cites W2050049123 @default.
• W2342703009 cites W2050736899 @default.
• W2342703009 cites W2051149546 @default.
• W2342703009 cites W2053495993 @default.
• W2342703009 cites W2055859583 @default.
• W2342703009 cites W2065519715 @default.
• W2342703009 cites W2078617761 @default.
• W2342703009 cites W2090429649 @default.
• W2342703009 cites W2104010513 @default.
• W2342703009 cites W2108188100 @default.
• W2342703009 cites W2132916293 @default.
• W2342703009 cites W2135151730 @default.
• W2342703009 cites W2137118785 @default.
• W2342703009 cites W2137649483 @default.
• W2342703009 cites W2140328948 @default.
• W2342703009 cites W2151165319 @default.
• W2342703009 cites W2154461811 @default.
• W2342703009 cites W2159330672 @default.
• W2342703009 cites W2162361061 @default.
• W2342703009 cites W2320147764 @default.
• W2342703009 cites W4248378708 @default.
• W2342703009 doi "https://doi.org/10.1152/ajprenal.00251.2015" @default.
• W2342703009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26377795" @default.
• W2342703009 hasPublicationYear "2015" @default.
• W2342703009 type Work @default.
• W2342703009 sameAs 2342703009 @default.
• W2342703009 citedByCount "11" @default.
• W2342703009 countsByYear W23427030092015 @default.
• W2342703009 countsByYear W23427030092016 @default.
• W2342703009 countsByYear W23427030092019 @default.
• W2342703009 countsByYear W23427030092020 @default.
• W2342703009 countsByYear W23427030092021 @default.
• W2342703009 countsByYear W23427030092022 @default.
• W2342703009 crossrefType "journal-article" @default.
• W2342703009 hasAuthorship W2342703009A5013151848 @default.
• W2342703009 hasAuthorship W2342703009A5021592381 @default.
• W2342703009 hasAuthorship W2342703009A5033449275 @default.
• W2342703009 hasAuthorship W2342703009A5034116139 @default.
• W2342703009 hasAuthorship W2342703009A5044459999 @default.
• W2342703009 hasAuthorship W2342703009A5047082140 @default.
• W2342703009 hasAuthorship W2342703009A5050687492 @default.
• W2342703009 hasAuthorship W2342703009A5057443708 @default.
• W2342703009 hasAuthorship W2342703009A5084723267 @default.
• W2342703009 hasAuthorship W2342703009A5085406568 @default.
• W2342703009 hasAuthorship W2342703009A5085491969 @default.
• W2342703009 hasAuthorship W2342703009A5090275275 @default.
• W2342703009 hasConcept C102230213 @default.
• W2342703009 hasConcept C104317684 @default.
• W2342703009 hasConcept C125929170 @default.
• W2342703009 hasConcept C141035611 @default.
• W2342703009 hasConcept C142613039 @default.
• W2342703009 hasConcept C15152581 @default.
• W2342703009 hasConcept C153911025 @default.
• W2342703009 hasConcept C173396325 @default.
• W2342703009 hasConcept C190232843 @default.
• W2342703009 hasConcept C203014093 @default.
• W2342703009 hasConcept C2522874641 @default.
• W2342703009 hasConcept C2779260011 @default.
• W2342703009 hasConcept C2779690655 @default.
• W2342703009 hasConcept C2780727368 @default.

• next