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• W2342873197 endingPage "481" @default.
• W2342873197 startingPage "469" @default.
• W2342873197 abstract "1. Intact rat epididymal fat-cells were incubated with 32Pi, and the intracellular proteins were separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. One of the separated bands of phosphorylated proteins had an apparent subunit mol.wt. of 42 000, which is the same as that of the alpha-subunit of the pyruvate dehydrogenase complex. By using a combination of subcellular fractionation, immunoprecipitation with antiserum raised against pyruvate dehydrogenase complex and two-dimensional electrophoresis it was apparent that the incorporation into alpha-subunits accounted for 35–45% of the total incorporation into this band of phosphoproteins. 2. The increase in the initial activity of pyruvate dehydrogenase that follows brief exposure of fat-cells to insulin was shown to be associated with a decrease in the steady-state incorporation of 32P into the alpha-subunits of pyruvate dehydrogenase. 3. Tryptic peptide analysis of pyruvate dehydrogenase [32P]phosphate, labelled in intact fat-cells, indicated that three serine residues on the alpha-subunit were phosphorylated, corresponding to the three sites phosphorylated when purified pig heart pyruvate dehydrogenase was incubated with [gamma-32P]ATP. The relative phosphorylation of all three serine residues appeared to be similar in 32P-labelled alpha-subunits in both control and insulin-treated fat-cells." @default.
• W2342873197 created "2016-06-24" @default.
• W2342873197 creator A5062935914 @default.
• W2342873197 creator A5076497150 @default.
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• W2342873197 date "1980-11-15" @default.
• W2342873197 modified "2023-10-16" @default.
• W2342873197 title "Studies on the incorporation of [32P]phosphate into pyruvate dehydrogenase in intact rat fat-cells. Effects of insulin" @default.
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• W2342873197 doi "https://doi.org/10.1042/bj1920469" @default.
• W2342873197 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1162361" @default.
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• W2342873197 hasPublicationYear "1980" @default.
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