Matches in SemOpenAlex for { <https://semopenalex.org/work/W2342953417> ?p ?o ?g. }
- W2342953417 endingPage "3812" @default.
- W2342953417 startingPage "3802" @default.
- W2342953417 abstract "Abstract Sirtuins participate in sensing nutrient availability and directing metabolic activity to match energy needs with energy production and consumption. However, the pivotal targets for sirtuins in cancer are mainly unknown. In this study, we identify the M2 isoform of pyruvate kinase (PKM2) as a critical target of the sirtuin SIRT2 implicated in cancer. PKM2 directs the synthesis of pyruvate and acetyl-CoA, the latter of which is transported to mitochondria for use in the Krebs cycle to generate ATP. Enabled by a shotgun mass spectrometry analysis founded on tissue culture models, we identified a candidate SIRT2 deacetylation target at PKM2 lysine 305 (K305). Biochemical experiments including site-directed mutants that mimicked constitutive acetylation suggested that acetylation reduced PKM2 activity by preventing tetramerization to the active enzymatic form. Notably, ectopic overexpression of a deacetylated PKM2 mutant in Sirt2-deficient mammary tumor cells altered glucose metabolism and inhibited malignant growth. Taken together, our results argued that loss of SIRT2 function in cancer cells reprograms their glycolytic metabolism via PKM2 regulation, partially explaining the tumor-permissive phenotype of mice lacking Sirt2. Cancer Res; 76(13); 3802–12. ©2016 AACR." @default.
- W2342953417 created "2016-06-24" @default.
- W2342953417 creator A5032930499 @default.
- W2342953417 creator A5035159364 @default.
- W2342953417 creator A5037843732 @default.
- W2342953417 creator A5043152010 @default.
- W2342953417 creator A5048521624 @default.
- W2342953417 creator A5064751624 @default.
- W2342953417 creator A5070977547 @default.
- W2342953417 creator A5072740842 @default.
- W2342953417 creator A5073238361 @default.
- W2342953417 creator A5074458765 @default.
- W2342953417 creator A5077861885 @default.
- W2342953417 creator A5081343311 @default.
- W2342953417 creator A5084540086 @default.
- W2342953417 creator A5087920969 @default.
- W2342953417 date "2016-06-30" @default.
- W2342953417 modified "2023-10-11" @default.
- W2342953417 title "SIRT2-Mediated Deacetylation and Tetramerization of Pyruvate Kinase Directs Glycolysis and Tumor Growth" @default.
- W2342953417 cites W1848671119 @default.
- W2342953417 cites W1969836970 @default.
- W2342953417 cites W1972899801 @default.
- W2342953417 cites W1977043983 @default.
- W2342953417 cites W1987376048 @default.
- W2342953417 cites W1991020970 @default.
- W2342953417 cites W1993350172 @default.
- W2342953417 cites W1995042750 @default.
- W2342953417 cites W1996395636 @default.
- W2342953417 cites W1998974491 @default.
- W2342953417 cites W2003005056 @default.
- W2342953417 cites W2008037894 @default.
- W2342953417 cites W2010586937 @default.
- W2342953417 cites W2016670036 @default.
- W2342953417 cites W2027598957 @default.
- W2342953417 cites W2027850495 @default.
- W2342953417 cites W2029142939 @default.
- W2342953417 cites W2029255902 @default.
- W2342953417 cites W2034591470 @default.
- W2342953417 cites W2037723157 @default.
- W2342953417 cites W2039374791 @default.
- W2342953417 cites W2044784487 @default.
- W2342953417 cites W2049385704 @default.
- W2342953417 cites W2051509673 @default.
- W2342953417 cites W2059251972 @default.
- W2342953417 cites W2060814375 @default.
- W2342953417 cites W2073848428 @default.
- W2342953417 cites W2078023359 @default.
- W2342953417 cites W2083161952 @default.
- W2342953417 cites W2089551665 @default.
- W2342953417 cites W2090345572 @default.
- W2342953417 cites W2100260504 @default.
- W2342953417 cites W2102322990 @default.
- W2342953417 cites W2117674849 @default.
- W2342953417 cites W2130335333 @default.
- W2342953417 cites W2133573972 @default.
- W2342953417 cites W2145755327 @default.
- W2342953417 cites W2163958526 @default.
- W2342953417 cites W2167592268 @default.
- W2342953417 cites W2168921982 @default.
- W2342953417 cites W2258580992 @default.
- W2342953417 doi "https://doi.org/10.1158/0008-5472.can-15-2498" @default.
- W2342953417 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4930699" @default.
- W2342953417 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27197174" @default.
- W2342953417 hasPublicationYear "2016" @default.
- W2342953417 type Work @default.
- W2342953417 sameAs 2342953417 @default.
- W2342953417 citedByCount "87" @default.
- W2342953417 countsByYear W23429534172016 @default.
- W2342953417 countsByYear W23429534172017 @default.
- W2342953417 countsByYear W23429534172018 @default.
- W2342953417 countsByYear W23429534172019 @default.
- W2342953417 countsByYear W23429534172020 @default.
- W2342953417 countsByYear W23429534172021 @default.
- W2342953417 countsByYear W23429534172022 @default.
- W2342953417 countsByYear W23429534172023 @default.
- W2342953417 crossrefType "journal-article" @default.
- W2342953417 hasAuthorship W2342953417A5032930499 @default.
- W2342953417 hasAuthorship W2342953417A5035159364 @default.
- W2342953417 hasAuthorship W2342953417A5037843732 @default.
- W2342953417 hasAuthorship W2342953417A5043152010 @default.
- W2342953417 hasAuthorship W2342953417A5048521624 @default.
- W2342953417 hasAuthorship W2342953417A5064751624 @default.
- W2342953417 hasAuthorship W2342953417A5070977547 @default.
- W2342953417 hasAuthorship W2342953417A5072740842 @default.
- W2342953417 hasAuthorship W2342953417A5073238361 @default.
- W2342953417 hasAuthorship W2342953417A5074458765 @default.
- W2342953417 hasAuthorship W2342953417A5077861885 @default.
- W2342953417 hasAuthorship W2342953417A5081343311 @default.
- W2342953417 hasAuthorship W2342953417A5084540086 @default.
- W2342953417 hasAuthorship W2342953417A5087920969 @default.
- W2342953417 hasBestOaLocation W23429534171 @default.
- W2342953417 hasConcept C104317684 @default.
- W2342953417 hasConcept C10730799 @default.
- W2342953417 hasConcept C109156525 @default.
- W2342953417 hasConcept C119157956 @default.
- W2342953417 hasConcept C185592680 @default.
- W2342953417 hasConcept C199848840 @default.
- W2342953417 hasConcept C20251656 @default.