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- W2342969385 abstract "Molecular testing can be performed on different specimen types. DNA- and RNA-based ancillary studies play an important role in identifying molecular portraits of breast carcinoma and in influencing routine therapies. Preanalytical factors coupled with differences in chemical and physical conditions have a significant impact on the quality of the nucleic acids. Although there are different types of fixative for surgical specimens in breast pathology, the 10 % neutral buffered formalin (NBF) is the preferred and most widely used fixative, which most molecular assays have been optimized for. Among different molecular testing methods, in situ hybridization (ISH) and reverse-transcription polymerase chain reaction (PCR) assays are more commonly used. However, next generation sequencing (NGS) set the pace in changing our understanding of the molecular traits of tumors at the genomic, transcriptomic, and epigenetic levels and is rapidly gaining pace in clinical testing." @default.
- W2342969385 created "2016-06-24" @default.
- W2342969385 creator A5035868494 @default.
- W2342969385 creator A5074776852 @default.
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- W2342969385 date "2015-01-01" @default.
- W2342969385 modified "2023-10-03" @default.
- W2342969385 title "Specimens for Molecular Testing in Breast Cancer" @default.
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- W2342969385 doi "https://doi.org/10.1007/978-1-4939-2886-6_19" @default.
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