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- W2343286005 abstract "Objective: To assess the antioxidant and anticancer activity of crude ethyl acetate extract from Streptomyces cavouresis KU-V39 isolated from vermicompost. Methods: To determine its antioxidant activity by total phenol and flavonoid content, Hydrogen peroxide radical scavenging activity and Ferrous reducing power assay. To determine in vitro cytotoxicity, various concentrations of extract was tested on HeLa cell line by 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Fourier transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectroscopy (GC-MS) have been carried out to investigate the bioactive compound of crude extract. Results: The Phenol and flavonoid content of crude extract was found to be 20.24 and 26.60 mg /g metabolite. Isolate KU-V39 exhibited H 2O 2 scavenging activity at IC 50 value is 42.35 ± 0.75 μg/ml. The ferrous reducing power assay has higher absorbance value which indicates the high antioxidant capacity of the extracts. The analysis of FT-IR spectroscopy indicate presents of Phenol, carboxylic acid, alkanes, nitro and aliphatic amines functional groups Conclusion: This spectral study clearly proved that the vermicompost derived Streptomyces with extra cellular metabolism showed promising antioxidant and anticancer activity. . The GC – MS analysis revealed that presence of Pentanoic acid, L-Proline, Lysine, Erucic acid, isosteviol, Pentadecanoic acid, Phthalic acid, Hexadecanoic acid, Octadecenoic acid, Dichloroacetic acid, Cyclohexanecarboxylic acid and Pyrrolo pyrazine derivative." @default.
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- W2343286005 date "2014-01-01" @default.
- W2343286005 modified "2023-09-27" @default.
- W2343286005 title "SPECTROSCOPIC ANALYSIS OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES CAVOURESIS KUV39: EVALUATION OF ANTIOXIDANT AND CYTOTOXICITY ACTIVITY Original Article" @default.
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