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- W2343329114 abstract "A multiplex RT-PCR(mRT-PCR) system was established for simultaneous detection on Zucchini yellow mosaic virus(ZYMV),Watermelon mosaic virus(WMV),Tobacco mosaic virus(TMV),Squash mo-saic virus(SqMV)and Cucumber mosaic virus(CMV)infected watermelon by using five sets of specific primers designed on the basis of conserved sequences of the 5 viruses. The concentrations of the primers, Mg2+,Taq DNA polymerase and dNTPs were examined and the PCR conditions including annealing temperature and amplification cycles were optimized. The results showed that expected fragments of 542 bp(ZYMV),485 bp(WMV),410 bp(TMV), 354 bp(SqMV) and 293 bp(CMV)were amplified and the mRT-PCR system was successfully established and the multiplex RT-PCR had been applied in practice, provided a simple, rapid and economic method for simultaneous detection of the five watermelon viruses." @default.
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- W2343329114 date "2010-01-01" @default.
- W2343329114 modified "2023-09-23" @default.
- W2343329114 title "Establishment and application of multiplex RT-PCR for simultaneous detection of five watermelon viruses ZYMV, WMV, TMV, SqMV and CMV." @default.
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