FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2343600934> ?p ?o ?g. }

• W2343600934 endingPage "1775" @default.
• W2343600934 startingPage "1763" @default.
• W2343600934 abstract "Influx of extracellular Ca2+ plays a major role in the activation of contraction in fish cardiac cells. The relative contributions of Na+/Ca2+ exchange and L-type Ca2+ channels to Ca2+ influx are, however, unknown. Using a physiological action potential as the command pulse in voltage-clamped heart cells, we examined sarcolemmal Ca2+ influx through Na+/Ca2+ exchange and L-type Ca2+ channels in crucian carp (Carassius carassius L.) ventricular myocytes. When other cation conductances were blocked, a Ni2+-sensitive current with the characteristic voltage- and time-dependent properties of the Na+/Ca2+ exchange current could be distinguished. At the maximum overshoot voltage of the ventricular action potential (+40 mV; [Na+]i=10 mmol l-1), the density of the Na+/Ca2+ exchange current was 2.99+/-0.27 pA pF-1 for warm-acclimated fish (23 degrees C) and 2.38+/-0.42 pA pF-1 for cold-acclimated fish (4 degrees C) (means +/- s.e.m., N=5-6; not significantly different, P=0.26). The relative contributions of the Na+/Ca2+ exchanger and L-type Ca2+ channels to Ca2+ influx were estimated using two partly different methods. Integration of the Ni2+-sensitive Na+/Ca2+ exchange current and the verapamil- and Cd2+-sensitive L-type Ca2+ current suggests that, during the action potential, approximately one-third of the activating Ca2+ comes through Na+/Ca2+ exchange and approximately two-thirds through L-type Ca2+ channels. An alternative method of analysis, using the inward tail current as a measure of the total sarcolemmal Ca2+ flux from which the Ni2+-sensitive Na+/Ca2+ exchange current was subtracted to obtain the Ca2+ influx through the channels, suggests that L-type Ca2+ channels and Na+/Ca2+ exchange are almost equally important in the activation of contraction. Furthermore, the time course of cell shortening is not adequately explained by sarcolemmal Ca2+ influx through the channels alone, but is well approximated by the sum of Ca2+ influx through the channels and the exchanger. The present results indicate that reverse Na+/Ca2+ exchange in crucian carp ventricular myocytes has sufficient capacity to trigger contraction and suggest that the exchange current makes a significant contribution to contractile Ca2+ during the physiological action potential. The relative significance of channels and exchanger molecules in sarcolemmal Ca2+ entry into crucian carp ventricular myocytes was unaffected by thermal acclimation when determined at 22 degrees C." @default.
• W2343600934 created "2016-06-24" @default.
• W2343600934 creator A5062989015 @default.
• W2343600934 date "1999-07-01" @default.
• W2343600934 modified "2023-10-16" @default.
• W2343600934 title "Na+/Ca2+ exchange current in ventricular myocytes of fish heart: contribution to sarcolemmal Ca2+ influx" @default.
• W2343600934 cites W1980002181 @default.
• W2343600934 cites W1982884633 @default.
• W2343600934 cites W1995320987 @default.
• W2343600934 cites W2009667219 @default.
• W2343600934 cites W2014808188 @default.
• W2343600934 cites W2036377020 @default.
• W2343600934 cites W2042628491 @default.
• W2343600934 cites W2044527642 @default.
• W2343600934 cites W2069959839 @default.
• W2343600934 cites W2073598260 @default.
• W2343600934 cites W2094608685 @default.
• W2343600934 cites W2095979033 @default.
• W2343600934 cites W2102878961 @default.
• W2343600934 cites W2123433512 @default.
• W2343600934 cites W2125505135 @default.
• W2343600934 cites W2127934972 @default.
• W2343600934 cites W2219369075 @default.
• W2343600934 cites W2255702791 @default.
• W2343600934 cites W2397817760 @default.
• W2343600934 cites W2475247859 @default.
• W2343600934 cites W4251910526 @default.
• W2343600934 doi "https://doi.org/10.1242/jeb.202.13.1763" @default.
• W2343600934 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10359679" @default.
• W2343600934 hasPublicationYear "1999" @default.
• W2343600934 type Work @default.
• W2343600934 sameAs 2343600934 @default.
• W2343600934 citedByCount "58" @default.
• W2343600934 countsByYear W23436009342012 @default.
• W2343600934 countsByYear W23436009342013 @default.
• W2343600934 countsByYear W23436009342014 @default.
• W2343600934 countsByYear W23436009342015 @default.
• W2343600934 countsByYear W23436009342016 @default.
• W2343600934 countsByYear W23436009342017 @default.
• W2343600934 countsByYear W23436009342018 @default.
• W2343600934 countsByYear W23436009342019 @default.
• W2343600934 countsByYear W23436009342020 @default.
• W2343600934 countsByYear W23436009342021 @default.
• W2343600934 countsByYear W23436009342022 @default.
• W2343600934 crossrefType "journal-article" @default.
• W2343600934 hasAuthorship W2343600934A5062989015 @default.
• W2343600934 hasConcept C12554922 @default.
• W2343600934 hasConcept C126322002 @default.
• W2343600934 hasConcept C134018914 @default.
• W2343600934 hasConcept C146403970 @default.
• W2343600934 hasConcept C185263204 @default.
• W2343600934 hasConcept C185592680 @default.
• W2343600934 hasConcept C207200792 @default.
• W2343600934 hasConcept C47423411 @default.
• W2343600934 hasConcept C71924100 @default.
• W2343600934 hasConcept C86803240 @default.
• W2343600934 hasConceptScore W2343600934C12554922 @default.
• W2343600934 hasConceptScore W2343600934C126322002 @default.
• W2343600934 hasConceptScore W2343600934C134018914 @default.
• W2343600934 hasConceptScore W2343600934C146403970 @default.
• W2343600934 hasConceptScore W2343600934C185263204 @default.
• W2343600934 hasConceptScore W2343600934C185592680 @default.
• W2343600934 hasConceptScore W2343600934C207200792 @default.
• W2343600934 hasConceptScore W2343600934C47423411 @default.
• W2343600934 hasConceptScore W2343600934C71924100 @default.
• W2343600934 hasConceptScore W2343600934C86803240 @default.
• W2343600934 hasIssue "13" @default.
• W2343600934 hasLocation W23436009341 @default.
• W2343600934 hasLocation W23436009342 @default.
• W2343600934 hasOpenAccess W2343600934 @default.
• W2343600934 hasPrimaryLocation W23436009341 @default.
• W2343600934 hasRelatedWork W2033129782 @default.
• W2343600934 hasRelatedWork W2049712442 @default.
• W2343600934 hasRelatedWork W2078280030 @default.
• W2343600934 hasRelatedWork W2091968002 @default.
• W2343600934 hasRelatedWork W2115081597 @default.
• W2343600934 hasRelatedWork W2157651064 @default.
• W2343600934 hasRelatedWork W2379110061 @default.
• W2343600934 hasRelatedWork W3151887034 @default.
• W2343600934 hasRelatedWork W4313461941 @default.
• W2343600934 hasRelatedWork W1975131962 @default.
• W2343600934 hasVolume "202" @default.
• W2343600934 isParatext "false" @default.
• W2343600934 isRetracted "false" @default.
• W2343600934 magId "2343600934" @default.
• W2343600934 workType "article" @default.