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• W2343628044 abstract "A new method is developed for efficient delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetic nanoparticle complex on the surface of cells with subsequent nanosecond laser pulse generating stress waves in transfection chamber, which is able to permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been shown using siRNA inducing cell apoptosis, combination of these two physical factors increased the efficiency of three different human carcinoma cells transfection to 93%, 89%, and 84%, for HeLa (cervical carcinoma), MCF-7 (breast carcinoma), and UCI-107 (ovarian carcinoma) cells, respectively. This new physical method of siRNA delivery may have therefore far reaching applications in biotechnology and functional genomics." @default.
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• W2343628044 date "2016-04-27" @default.
• W2343628044 modified "2023-09-27" @default.
• W2343628044 title "Silencing by blasting: combination of laser pulse induced stress waves and magnetophoresis for siRNA delivery" @default.
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• W2343628044 doi "https://doi.org/10.1088/1612-2011/13/6/065601" @default.
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