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- W2343701238 abstract "Anisakids nematodes from Pacific cod (Gadus macrocephalus) in Korea were investigated and their molecular identification was conducted, to assess the epidemiological role of Pacific cod in human anisakidosis in Korea. Totally 238 Pacific cod were caught from 5 different areas around Korean peninsula. Fish were dissected and carefully examined for collecting nematodes. PCR-RFLP and the subsequent sequencing were conducted for molecular identification of those nematodes. A high prevalence of infection (193/238, 81.1%) in Pacific cod was observed, and 1694 nematodes were collected. 79.1% (1340/1694) of the nematodes were found freely in the body cavity of Pacific cod, and the rest of them (20.9%, 354/1694) were in the digestive tract or attached to other organs. PCR-RFLP analysis using HinfI and RsaI restriction enzymes revealed 3 different banding patterns corresponding to Anisakis pegreffii, Hysterothylacium aduncum and hybrid genotype (Anisakis simplex × A. pegreffii), respectively. Of 1694 nematodes, 1280 (75.6%) were identified as A. pegreffii and 406 (24.0%) were H. aduncum. A. pegreffii occupied 84.0% (1125/1340) of the nematodes in the body cavity and 40.0% (132/330) of them in the digestive tract, but no nematodes were found in Pacific cod muscles." @default.
- W2343701238 created "2016-06-24" @default.
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- W2343701238 date "2016-11-01" @default.
- W2343701238 modified "2023-10-14" @default.
- W2343701238 title "Occurrence and molecular identification of anisakid nematodes isolated from Pacific cod ( Gadus macrocephalus ) caught off Korea" @default.
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- W2343701238 doi "https://doi.org/10.1016/j.foodcont.2016.04.031" @default.
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