Matches in SemOpenAlex for { <https://semopenalex.org/work/W2343746546> ?p ?o ?g. }
- W2343746546 endingPage "89" @default.
- W2343746546 startingPage "83" @default.
- W2343746546 abstract "To improve the thermostability of xylanase XynZF-2 from Aspergillus niger XZ-3S, a disulfide bridge was introduced in the N-terminal domains by site-directed mutagenesis (V1C and E27C). Simultaneously, the active sites of XynZF-2 were predicted by bioinformatics software and verified by site-directed mutagenesis (E103D and E194D). The mutated active sites xynED- and the mutated disulfide bridge xynDC-encoding genes were constructed and expressed in Escherichia coli BL21 (DE3). Compared to the native xylanase, it was found that the residual activity of the mutated XynED was 0.17%. The optimum temperature of the variant XynDC was increased to 45°C from 40°C of XynZF-2. After treatment at 40°C for 60 min, the variant XynDC retained 66.77% of their original activity, while the XynZF-2 retained about 44.36% residual activity. t1/245°C of the variant XynDC also increased from 7 min to 14 min. The results of the mutated xylanases indicated that the active center of XynZF-2 mainly consisted of two catalytic residues (Glu103 and Glu194), and the introduction of a disulfide bridge in the N-terminal domains can improve the thermostability of XynZF-2." @default.
- W2343746546 created "2016-06-24" @default.
- W2343746546 creator A5002441411 @default.
- W2343746546 creator A5030599421 @default.
- W2343746546 creator A5045492537 @default.
- W2343746546 creator A5088408496 @default.
- W2343746546 creator A5089296571 @default.
- W2343746546 date "2016-01-01" @default.
- W2343746546 modified "2023-10-15" @default.
- W2343746546 title "Exploration of a N-terminal disulfide bridge to improve the thermostability of a GH11 xylanase from <i>Aspergillus niger</i>" @default.
- W2343746546 cites W1495442199 @default.
- W2343746546 cites W1531788433 @default.
- W2343746546 cites W1917818832 @default.
- W2343746546 cites W1966627397 @default.
- W2343746546 cites W1966980235 @default.
- W2343746546 cites W1982370265 @default.
- W2343746546 cites W1987622888 @default.
- W2343746546 cites W1988989351 @default.
- W2343746546 cites W2003139549 @default.
- W2343746546 cites W2008560925 @default.
- W2343746546 cites W2009330706 @default.
- W2343746546 cites W2009555092 @default.
- W2343746546 cites W2015943251 @default.
- W2343746546 cites W2016202530 @default.
- W2343746546 cites W2025026243 @default.
- W2343746546 cites W2029268391 @default.
- W2343746546 cites W2031196168 @default.
- W2343746546 cites W2032898089 @default.
- W2343746546 cites W2033684728 @default.
- W2343746546 cites W2042405246 @default.
- W2343746546 cites W2044195468 @default.
- W2343746546 cites W2049432570 @default.
- W2343746546 cites W2051940969 @default.
- W2343746546 cites W2057984012 @default.
- W2343746546 cites W2064424336 @default.
- W2343746546 cites W2073126822 @default.
- W2343746546 cites W2086924109 @default.
- W2343746546 cites W2110954443 @default.
- W2343746546 cites W2122496979 @default.
- W2343746546 cites W2136696778 @default.
- W2343746546 cites W2138270807 @default.
- W2343746546 cites W2144884182 @default.
- W2343746546 cites W2152756549 @default.
- W2343746546 cites W2154914432 @default.
- W2343746546 doi "https://doi.org/10.2323/jgam.62.83" @default.
- W2343746546 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27118076" @default.
- W2343746546 hasPublicationYear "2016" @default.
- W2343746546 type Work @default.
- W2343746546 sameAs 2343746546 @default.
- W2343746546 citedByCount "5" @default.
- W2343746546 countsByYear W23437465462017 @default.
- W2343746546 countsByYear W23437465462019 @default.
- W2343746546 countsByYear W23437465462020 @default.
- W2343746546 crossrefType "journal-article" @default.
- W2343746546 hasAuthorship W2343746546A5002441411 @default.
- W2343746546 hasAuthorship W2343746546A5030599421 @default.
- W2343746546 hasAuthorship W2343746546A5045492537 @default.
- W2343746546 hasAuthorship W2343746546A5088408496 @default.
- W2343746546 hasAuthorship W2343746546A5089296571 @default.
- W2343746546 hasBestOaLocation W23437465461 @default.
- W2343746546 hasConcept C103408237 @default.
- W2343746546 hasConcept C104317684 @default.
- W2343746546 hasConcept C143065580 @default.
- W2343746546 hasConcept C16318435 @default.
- W2343746546 hasConcept C181199279 @default.
- W2343746546 hasConcept C185592680 @default.
- W2343746546 hasConcept C27256138 @default.
- W2343746546 hasConcept C2775859485 @default.
- W2343746546 hasConcept C2775960407 @default.
- W2343746546 hasConcept C2781216036 @default.
- W2343746546 hasConcept C41183919 @default.
- W2343746546 hasConcept C501734568 @default.
- W2343746546 hasConcept C547475151 @default.
- W2343746546 hasConcept C55493867 @default.
- W2343746546 hasConcept C71240020 @default.
- W2343746546 hasConceptScore W2343746546C103408237 @default.
- W2343746546 hasConceptScore W2343746546C104317684 @default.
- W2343746546 hasConceptScore W2343746546C143065580 @default.
- W2343746546 hasConceptScore W2343746546C16318435 @default.
- W2343746546 hasConceptScore W2343746546C181199279 @default.
- W2343746546 hasConceptScore W2343746546C185592680 @default.
- W2343746546 hasConceptScore W2343746546C27256138 @default.
- W2343746546 hasConceptScore W2343746546C2775859485 @default.
- W2343746546 hasConceptScore W2343746546C2775960407 @default.
- W2343746546 hasConceptScore W2343746546C2781216036 @default.
- W2343746546 hasConceptScore W2343746546C41183919 @default.
- W2343746546 hasConceptScore W2343746546C501734568 @default.
- W2343746546 hasConceptScore W2343746546C547475151 @default.
- W2343746546 hasConceptScore W2343746546C55493867 @default.
- W2343746546 hasConceptScore W2343746546C71240020 @default.
- W2343746546 hasIssue "2" @default.
- W2343746546 hasLocation W23437465461 @default.
- W2343746546 hasLocation W23437465462 @default.
- W2343746546 hasOpenAccess W2343746546 @default.
- W2343746546 hasPrimaryLocation W23437465461 @default.
- W2343746546 hasRelatedWork W1970241958 @default.
- W2343746546 hasRelatedWork W2016202530 @default.
- W2343746546 hasRelatedWork W2033684728 @default.