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• W2343971948 abstract "Abstract Ion homeostasis plays a central role in polarisation and polar growth. In several cell types ion channels are controlled by reactive oxygen species ( ROS ). One of the most important cells in the plant life cycle is the male gametophyte, which grows under the tight control of both ion fluxes and ROS balance. The precise relationship between these two factors in pollen tubes has not been completely elucidated, and in pollen grains it has never been studied to date. In the present study we used a simple model – protoplasts obtained from lily pollen grains at the early germination stage – to reveal the effect of H 2 O 2 on cation fluxes crucial for pollen germination. Here we present direct evidence for two ROS ‐sensitive currents on the pollen grain plasma membrane: the hyperpolarisation‐activated calcium current, which is strongly enhanced by H 2 O 2 , and the outward potassium current, which is modestly enhanced by H 2 O 2 . We used low concentrations of H 2 O 2 that do not cause an intracellular oxidative burst and do not damage cells, as demonstrated with fluorescent staining." @default.
• W2343971948 created "2016-06-24" @default.
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• W2343971948 date "2016-05-18" @default.
• W2343971948 modified "2023-10-06" @default.
• W2343971948 title "Hydrogen peroxide affects ion channels in lily pollen grain protoplasts" @default.
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• W2343971948 doi "https://doi.org/10.1111/plb.12470" @default.
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